Back to Search
Start Over
MALDI-TOF-MS reveals differential N-linked plasma- and IgG-glycosylation profiles between mothers and their newborns
- Source :
- Scientific Reports, Scientific reports, 6. Nature Publishing Group, Scientific Reports, 6:34001. Nature Publishing Group
- Publication Year :
- 2016
-
Abstract
- During pregnancy, the mother provides multiple nutrients and substances to the foetus, with maternal immunoglobulin G (IgG) being actively transported to the foetus. Newborns depend on maternal IgG for immune-protection in their first months. The glycosylation of IgG has been shown to influence its dynamics, e.g. receptor binding. While minor differences in IgG glycosylation have been found between IgG derived from maternal blood and umbilical cord blood (UC) of newborn children, the differential glycosylation of maternal and UC plasma has hitherto not been studied. Here, we studied the N-glycosylation of IgG and total plasma proteome of both maternal and UC plasma of 42 pairs of mothers and newborn children. A total of 37 N-glycans were quantified for IgG and 45 for the total plasma N-glycome (TPNG). The study showed slightly higher levels of galactosylation for UC IgG than maternal IgG, confirming previous results, as well as lower bisection and sialylation. Furthermore, the TPNG results showed lower values for galactosylation and sialylation and higher values for fucosylation in the UC plasma. In conclusion, this study presents some novel insights into IgG glycosylation differences as well as the first broad overview of the differential plasma glycosylation between mothers and newborns.
- Subjects :
- 0301 basic medicine
Glycosylation
FC-GAMMA-RIII
NORMAL-PREGNANCY
Umbilical cord
Article
Immunoglobulin G
Andrology
03 medical and health sciences
chemistry.chemical_compound
PROTEIN-TRANSPORT
Immune system
BINDING
medicine
Receptor
FETAL
Fucosylation
Pregnancy
Fetus
PLACENTAL TRANSPORT
Multidisciplinary
RECEPTOR
biology
Chemistry
SIALIC-ACID ESTERIFICATION
medicine.disease
carbohydrates (lipids)
030104 developmental biology
medicine.anatomical_structure
Immunology
biology.protein
IMMUNOGLOBULIN-G
IMMUNE-SYSTEM
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific reports, 6. Nature Publishing Group, Scientific Reports, 6:34001. Nature Publishing Group
- Accession number :
- edsair.doi.dedup.....ddae95819a6c9291a8c2f1640fe7fdf2