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Expression of Cre Recombinase in Pigment Cells
- Source :
- Pigment Cell Research. 15:305-309
- Publication Year :
- 2002
- Publisher :
- Wiley, 2002.
-
Abstract
- Conditional gene targeting using the Cre/loxP system enables specific deletion of a gene in a tissue of interest. For application of Cre-mediated recombination in pigment cells, Cre expression has to be targeted to pigment cells in transgenic mice. So far, no pigment cell-specific Cre transgenic line has been reported and we present and discuss our first results on use of Cre recombinase in pigment cells. A construct was generated where Cre recombinase is controlled by the promoter of the mouse dopachrome tautomerase (Dct) gene. The construct was functionally tested in vitro and introduced into mice. Following breeding to two reporter mouse strains, we detected Cre recombinase activity in telencephalon, melanoblasts, and retinal pigment epithelium (RPE). Our data demonstrate the feasibility of pigment cell-specific Cre/loxP-mediated recombination.
- Subjects :
- Male
Genetically modified mouse
Recombinant Fusion Proteins
Transgene
Green Fluorescent Proteins
Clinical Biochemistry
Cre recombinase
Mice, Transgenic
Plant Science
Biology
Melanocyte
Mice
Viral Proteins
medicine
Animals
Pigment Epithelium of Eye
Promoter Regions, Genetic
Gene
Cells, Cultured
Melanins
Retinal pigment epithelium
Integrases
Gene targeting
Cell Biology
Molecular biology
Intramolecular Oxidoreductases
Luminescent Proteins
medicine.anatomical_structure
Gene Targeting
Codon, Terminator
Melanocytes
Female
sense organs
Agronomy and Crop Science
Dopachrome tautomerase
Developmental Biology
Subjects
Details
- ISSN :
- 08935785
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Pigment Cell Research
- Accession number :
- edsair.doi.dedup.....dd89d007d575913dba33e39ffc59f87c