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Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites
- Source :
- Peng, B, Klausen, C, Campbell, L, Leung, P C K, Horne, A W & Bedaiwy, M A 2016, ' Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites ', Fertility and Sterility . https://doi.org/10.1016/j.fertnstert.2016.02.003
- Publication Year :
- 2015
-
Abstract
- OBJECTIVE: To investigate whether gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) are expressed at tubal ectopic pregnancy sites, and to study the potential role of GnRH signaling in regulating immortalized human trophoblast cell viability.DESIGN: Immunohistochemical and experimental studies.SETTING: Academic research laboratory.PATIENT(S): Fallopian tube implantation sites (n = 25) were collected from women with ectopic pregnancy. First-trimester human placenta biopsies (n = 5) were obtained from elective terminations of pregnancy.INTERVENTION(S): None.MAIN OUTCOME MEASURE(S): GnRH and GnRHR expression was examined by means of immunohistochemistry and histoscoring. Trophoblastic BeWo choriocarcinoma and immortalized extravillous trophoblast (HTR-8/SVneo) cell viability was examined by means of cell counting after incubation with GnRH and/or GnRH antagonist (Antide).RESULT(S): GnRH and GnRHR immunoreactivity was detected in cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast in all women with tubal pregnancy. GnRH immunoreactivity was higher and GnRHR immunoreactivity lower in syncytiotrophoblast compared with cytotrophoblast. GnRH and GnRHR immunoreactivity was detected in adjacent fallopian tube epithelium. Whereas neither GnRH nor Antide altered HTR-8/SVneo cell viability, treatment with GnRH significantly increased the overall cell viability of BeWo cells at 48 and 72 hours, and these effects were abolished by pretreatment with Antide.CONCLUSION(S): GnRH and GnRHR are expressed in trophoblast cell populations and fallopian tube epithelium at tubal ectopic pregnancy sites. GnRH increases BeWo cell viability, an effect mediated by the GnRHR. Further work is required to investigate the potential role of GnRH signaling in ectopic pregnancy.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
endocrine system
Cell Survival
Gonadotropin-releasing hormone
Biology
Gonadotropin-Releasing Hormone
03 medical and health sciences
0302 clinical medicine
Syncytiotrophoblast
Pregnancy
Internal medicine
Cell Line, Tumor
medicine
Humans
Embryo Implantation
reproductive and urinary physiology
Fallopian Tubes
Cell Line, Transformed
030219 obstetrics & reproductive medicine
Cytotrophoblast
Ectopic pregnancy
GNRHR
Obstetrics and Gynecology
Trophoblast
Gene Expression Regulation, Developmental
medicine.disease
female genital diseases and pregnancy complications
Trophoblasts
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Reproductive Medicine
embryonic structures
Female
Pregnancy, Tubal
Gonadotropin-releasing hormone receptor
hormones, hormone substitutes, and hormone antagonists
Receptors, LHRH
Fallopian tube
Subjects
Details
- ISSN :
- 15565653
- Volume :
- 105
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Fertility and sterility
- Accession number :
- edsair.doi.dedup.....dd764c7470f7fbb684580246016199c6