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Interleukin-5 expressed by a recombinant virus vector enhances specific mucosal IgA responsesin vivo
- Source :
- European Journal of Immunology. 23:3141-3145
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- Several in vitro studies have shown that murine interleukin-5 (mIL-5) enhances IgA production by activated mucosal B cells. To date, however, there is no evidence that this factor significantly up-regulates mucosal IgA responses in vivo. Here, we show that expression of the gene for mIL-5 in a recombinant vaccinia virus vector markedly increases IgA responses to co-expressed heterologous antigen in the lungs of mice given intranasal inocula of the virus. The elevated local IgA responses to vectors expressing mIL-5 peaked at a fourfold higher level than those elicited by control virus at 14 days after infection and were sustained for at least 4 weeks. Increased IgA responses were abrogated in mice treated with monoclonal antibody against mIL-5 and were not detected in systemic lymphoid tissue. No enhancement of specific IgG levels was found either locally or systemically. Our results indicate that mIL-5 selectively enhances the development of mucosal IgA responses in vivo and suggest that expression of this factor in mucosal vaccine vectors may stimulate local immune reactivity.
- Subjects :
- medicine.drug_class
medicine.medical_treatment
Genetic Vectors
Immunology
Heterologous
Vaccinia virus
Biology
Monoclonal antibody
Recombinant virus
Virus
Mice
Antigen
In vivo
medicine
Animals
Immunology and Allergy
Lung
Interleukin 5
Mucous Membrane
Antibodies, Monoclonal
Molecular biology
Recombinant Proteins
Cytokine
Immunoglobulin A, Secretory
Mice, Inbred CBA
Immunization
Interleukin-5
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....dd492d7c1babfab71c28dae7a2fb2fcd