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Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization
- Source :
- Nature. 377(6549)
- Publication Year :
- 1995
-
Abstract
- THE localization of oskar (osk) RNA to the posterior pole of the developing fruit fly (Drosophila) oocyte induces the assembly of pole plasm, causing development of the abdomen and germ line1,2. Failure to localize oskar RNA results in embryos that lack abdomen and germ cells. Conversely, mis-targeting of oskar RNA to the anterior of the oocyte causes formation of ectopic abdomen and germ cells at the anterior pole3. Maternal mutants that have reduced pole plasm activity produce sterile adults with normal abdominal development, suggesting that germ cells are more sensitive than abdomen to defects in pole plasm assembly4. Thus mutations in genes that reduce oskar RNA localization or activity can be recovered as viable sterile adults. In a screen for mutants defective in germ cell formation, we isolated nine alleles of the tropomyosin II gene5. Here we show that mutations in tropomyosin II (TmII) virtually abolish oskar RNA localization to the posterior pole, suggesting an involvement of the actin network in oskar RNA localization.
- Subjects :
- Cytoplasm
RNA localization
Tropomyosin
Biology
oskar
Gene interaction
Gene expression
medicine
Animals
Drosophila Proteins
RNA, Messenger
Genetics
Multidisciplinary
urogenital system
RNA
Cell Polarity
Proteins
RNA-Binding Proteins
Oocyte
Actins
Cell biology
medicine.anatomical_structure
Mutation
Pole plasm
Oocytes
Drosophila
Female
Germ cell
Subjects
Details
- ISSN :
- 00280836
- Volume :
- 377
- Issue :
- 6549
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....dd45e4ace7bb9dd1faa3a973ea990dbf