Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle

Made available in DSpace on 2018-11-28T03:24:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) ACSC There is evidence that regulation of follicle selection in cattle involves locally produced growth factors. In the present study, we investigated the expression of members of the fibroblast growth factor (FGF) 7 family during follicle deviation. The largest and second largest follicles were recovered during the second day of a synchronised follicle wave and the future dominant and future subordinate follicles were identified based on diameter and cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) mRNA levels in granulosa cells. Theca cells of the future dominant follicle contained less mRNA encoding FGF7 and FGF10 compared with those from the future subordinate follicle 2.5 days after ovulation, before a significant difference between the diameters of the future dominant and future subordinate follicles could be observed, but FGF22 mRNA levels did not change. Levels of mRNA encoding FGF receptors FGFR1B and FGFR2B in theca and granulosa cells, respectively, were lower in the future dominant follicle compared with the future subordinate follicle. Addition of FGF10 to granulosa cells in vitro significantly decreased oestradiol secretion, as well as CYP19A1, FSH receptor (FSHR) and insulin-like growth factor 1 receptor (IGF1R) mRNA abundance, whereas FGF22 had no effect. We conclude that FGF10 and FGFR2B expression is increased in the future subordinate follicle before morphological deviation, which may contribute to follicle selection. Univ Estadual Paulista, Dept Farmacol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil Univ Montreal, Fac Med Vet, Ctr Rech Reprod Anim, 3200 Sicotte, St Hyacinthe, PQ J2S 7C6, Canada Univ Fed Santa Maria, Lab Biotecnol & Reprod Anim, Ave Roraima 1000, BR-97105900 Santa Maria, RS, Brazil Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil Univ Estadual Paulista, Dept Farmacol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil Univ Estadual Paulista, Dept Fisiol, Inst Biociencias, BR-18618970 Sao Paulo, Brazil FAPESP: 2008/57365-2