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Early Protein Markers of Necrotizing Enterocolitis in Plasma of Preterm Pigs Exposed to Antibiotics
- Source :
- Jiang, Y-N, Muk, T, Stensballe, A, Nguyen, D N, Sangild, P T & Jiang, P-P 2020, ' Early Protein Markers of Necrotizing Enterocolitis in Plasma of Preterm Pigs Exposed to Antibiotics ', Frontiers in Immunology, vol. 11, 565862 . https://doi.org/10.3389/fimmu.2020.565862, Jiang, Y N, Muk, T, Stensballe, A, Nguyen, D N, Sangild, P T & Jiang, P P 2020, ' Early Protein Markers of Necrotizing Enterocolitis in Plasma of Preterm Pigs Exposed to Antibiotics ', Frontiers in Immunology, vol. 11, 565862 . https://doi.org/10.3389/fimmu.2020.565862, Frontiers in Immunology, Frontiers in Immunology, Vol 11 (2020)
- Publication Year :
- 2020
-
Abstract
- Background: Most hospitalized preterm infants receive antibiotics in the first days of life to prevent or treat infections. Short-term, early antibiotic treatment may also prevent the microbiota-dependent gut inflammatory disorder, necrotizing enterocolitis (NEC). It remains a challenge to predict NEC, and a few early blood diagnostic markers exist. Using preterm pigs as model for infants, blood parameters and plasma proteins affected by early progression of NEC were profiled in preterm pigs subjected to oral, systemic, or no antibiotics after preterm birth. Methods: Preterm newborn pigs were treated with saline (CON) or antibiotics (ampicillin, gentamicin, and metronidazole) given enterally (ENT) or parenterally (PAR), and fed formula for 4 days to induce variable microbiome-dependent sensitivities to NEC. The gut was collected for macroscopic scoring of NEC lesions and blood for hematology, blood biochemistry, and LC/MS-based plasma proteomics. Statistical modeling was applied to detect plasma proteins affected by NEC and/or antibiotics. Results: Analyzed across different antibiotic regimens, NEC progression was associated with altered blood parameters and abundance of 89 plasma proteins that were functionally involved in extracellular membrane destruction, lipid metabolism, coagulopathy, and acute phase response. Large NEC-related changes were observed in abundance of RBP4, FGA, AHSG, C5, PTPRG, and A-1-antichymotrypsin 2, indicating potential serving as early markers of NEC. Conversely, antibiotic treatment, independent of NEC, affected only 4 proteins with main differences found between ENT and CON pigs. Conclusion: Early postnatal development of NEC lesions is associated with marked plasma protein changes that may be used for early NEC diagnosis.
- Subjects :
- lcsh:Immunologic diseases. Allergy
Male
0301 basic medicine
lipid metabilism
medicine.medical_specialty
Swine
medicine.drug_class
Immunology
Antibiotics
Gastroenterology
antibiotics
03 medical and health sciences
0302 clinical medicine
proteomics
Enterocolitis, Necrotizing
Ampicillin
Internal medicine
medicine
Animals
Immunology and Allergy
Original Research
Hematology
ECM
business.industry
Acute-phase protein
Blood Proteins
necrotizing enterocolitis (NEC)
medicine.disease
Blood proteins
immunity
digestive system diseases
Metronidazole
030104 developmental biology
Animals, Newborn
Necrotizing enterocolitis
Female
Gentamicin
lcsh:RC581-607
business
Biomarkers
030215 immunology
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Jiang, Y-N, Muk, T, Stensballe, A, Nguyen, D N, Sangild, P T & Jiang, P-P 2020, ' Early Protein Markers of Necrotizing Enterocolitis in Plasma of Preterm Pigs Exposed to Antibiotics ', Frontiers in Immunology, vol. 11, 565862 . https://doi.org/10.3389/fimmu.2020.565862, Jiang, Y N, Muk, T, Stensballe, A, Nguyen, D N, Sangild, P T & Jiang, P P 2020, ' Early Protein Markers of Necrotizing Enterocolitis in Plasma of Preterm Pigs Exposed to Antibiotics ', Frontiers in Immunology, vol. 11, 565862 . https://doi.org/10.3389/fimmu.2020.565862, Frontiers in Immunology, Frontiers in Immunology, Vol 11 (2020)
- Accession number :
- edsair.doi.dedup.....dd3b8bcf56f4c6def875f6c855f5a6de