Inactivation and repair of polyvalent Staphylococcus phages after U.V.- or gamma-irradiation or acridine orange treatment. II. Influence of the growth of phage A-5 in various hosts on the change of its sensitivity

SummaryWe studied the influence of the growth of polyvalent Staphylococcus phage A/5 in eight bacterial hosts on the course of inactivation of the resultant phage lysates by u.v.- and gamma-radiation and by acridine orange. The starting titrations of all the phage stocks used were identical. The propagation of phage A/5 through various hosts affected the course of inactivation of the phage and, especially, its resistance to u.v.-radiation was increased. Propagation of phage A/5 through host strain B1 produces its resistance both to u.v.- and to gamma-radiation as well as to photodynamic inactivation, whereas the host strains producing the greatest sensitivity of phage A/5 to these three agents, differ in all cases.