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Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs
- Source :
- Stem Cell Research, Vol 50, Iss, Pp 102120-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.
- Subjects :
- 0301 basic medicine
Transcription activator-like effector nuclease
Cell Biology
General Medicine
Biology
Green fluorescent protein
Cell biology
03 medical and health sciences
Exon
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
lcsh:Biology (General)
chemistry
Puromycin
cardiovascular system
Coding region
Induced pluripotent stem cell
lcsh:QH301-705.5
Transcription factor
Gene
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 18735061
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Stem Cell Research
- Accession number :
- edsair.doi.dedup.....dd22c66b585ce8a482c3ae86b115b8ff