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Fluorescence fingerprints of oral bacteria
- Source :
- Journal of Biophotonics. 13
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- The rapid detection and identification of microorganisms is one of the most important factors in many cases of ill health. The purpose of this study was to determine the fluorescence characteristics of seven oral bacteria using emission spectra with the aim of distinguishing between the bacteria, and to compare fluorescence imaging methods for the direct assessment of oral bacteria. Fluorescence images of each bacterium were obtained under a 405-nm light source using a two-filter system. The emissions of all samples were measured with a fluorescence spectrometer. The complete fluorescence data set collected for each sample employed a three-dimensional data cube. The differences in the autofluorescence characteristics of the seven oral bacteria were determined by principal components analysis (PCA). The fluorescence images of the oral bacteria varied with the genus and the filter system. The three-dimensional excitation-emission matrix fluorescence spectra exhibited distinctive fluorescence features associated with intracellular fluorophores. The seven bacteria could be clearly differentiated on the PCA score plot. The findings of this study indicate that oral bacteria can be identified based on their autofluorescence characteristics. Fluorescence spectroscopy coupled with PCA can be used to detect and classify oral bacteria.
- Subjects :
- Fluorescence-lifetime imaging microscopy
Microorganism
General Physics and Astronomy
01 natural sciences
Fluorescence
General Biochemistry, Genetics and Molecular Biology
Fluorescence spectroscopy
010309 optics
Matrix (chemical analysis)
0103 physical sciences
General Materials Science
Fluorescent Dyes
Principal Component Analysis
Chromatography
Bacteria
biology
Chemistry
Optical Imaging
010401 analytical chemistry
General Engineering
General Chemistry
biology.organism_classification
0104 chemical sciences
Autofluorescence
Spectrometry, Fluorescence
Principal component analysis
Subjects
Details
- ISSN :
- 18640648 and 1864063X
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Biophotonics
- Accession number :
- edsair.doi.dedup.....dd168241c4677f9184e2c9ad591fc4d1