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Assessment of post-thawed ram sperm viability after incubation with seminal plasma
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2011
-
Abstract
- A suggested alternative to improve post-thawed ram semen quality is the addition of seminal plasma (SP). This is thought to be capable of improving sperm resistance to thermal shock, reverting cryocapacitation and helping sperm survival. The aim of this study was to evaluate the effect of frozen-thawed ram semen incubation with SP on mitochondrial activity, acrosomal membrane integrity, necrosis and apoptosis. Frozen/thawed semen was divided into two groups: the SP Group and the control group. After 0, 30 and 60 min, fluorescent probes were added to aliquots from each treatment group and evaluated using flow cytometry. There was no difference between treatment groups in almost all viability parameters evaluated, with exception of the apoptosis, which was found increased in SP group. The increase in incubation period resulted in a decreased percentage of sperm with high mitochondrial membrane potential and acrosomal membrane integrity and an increased percentage of necrotic and apoptotic sperm cells. In conclusion, the present study showed that addition of seminal plasma after thawing cryopreserved ram sperm had no identifiable beneficial effect on sperm quality.
- Subjects :
- Male
endocrine system
Cell Survival
Biomedical Engineering
Semen
Apoptosis
Biology
Cryopreservation
Incubation period
Biomaterials
Andrology
Semen quality
Animals
Acrosome
Incubation
Cells, Cultured
NECROSE
Membrane Potential, Mitochondrial
Transplantation
Sheep
urogenital system
Cell Biology
Acrosomal membrane
Sperm
Spermatozoa
Mitochondria
Immunology
Models, Animal
Subjects
Details
- ISSN :
- 15736814
- Volume :
- 14
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cell and tissue banking
- Accession number :
- edsair.doi.dedup.....dd10962df4d49a68292439d9a98ef873