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Rapid determination of plasmonic nanoparticle agglomeration status in blood
- Source :
- Biomaterials. 51
- Publication Year :
- 2014
-
Abstract
- Plasmonic nanomaterials as drug delivery or bio-imaging agents are typically introduced to biological systems through intravenous administration. However, the potential for agglomeration of nanoparticles in biological systems could dramatically affect their pharmacokinetic profile and toxic potential. Development of rapid screening methods to evaluate agglomeration is urgently needed to monitor the physical nature of nanoparticles as they are introduced into blood. Here, we establish novel methods using darkfield microscopy with hyperspectral detection (hsDFM), single particle inductively-coupled plasma mass spectrometry (spICP-MS), and confocal Raman microscopy (cRM) to discriminate gold nanoparticles (AuNPs) and their agglomerates in blood. Rich information about nanoparticle agglomeration in situ is provided by hsDFM monitoring of the plasmon resonance of primary nanoparticles and their agglomerates in whole blood; cRM is an effective complement to hsDFM to detect AuNP agglomerates in minimally manipulated samples. The AuNPs and the particle agglomerates were further distinguished in blood for the first time by quantification of particle mass using spICP-MS with excellent sensitivity and specificity. Furthermore, the agglomeration status of synthesized and commercial NPs incubated in blood was successfully assessed using the developed methods. Together, these complementary methods enable rapid determination of the agglomeration status of plasmonic nanomaterials in biological systems, specifically blood.
- Subjects :
- Microscopy
Materials science
Economies of agglomeration
Spectrophotometry, Atomic
Biophysics
Nanoparticle
Metal Nanoparticles
Bioengineering
Nanotechnology
Spectrum Analysis, Raman
Rats, Inbred F344
Article
Nanomaterials
Biomaterials
Blood
Mechanics of Materials
Colloidal gold
Drug delivery
Ceramics and Composites
Particle
Animals
Scattering, Radiation
Citrates
Surface plasmon resonance
Whole blood
Subjects
Details
- ISSN :
- 18785905
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....dcca1bcf5ec7441e83736556d5a349ad