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Direct Measurement of Free Radical Levels in the Brain After Cortical Ischemia Induced by Photothrombosis
- Source :
- Physiological Research. :853-860
- Publication Year :
- 2016
- Publisher :
- Institute of Physiology of the Czech Academy of Sciences, 2016.
-
Abstract
- Tissue ischemia is connected with the production of free radicals (FR). This study was designed to directly measure of the amount of FR in rat brains related to a photothrombotic ischemic event shortly after establishing the lesion. A model of left hemisphere photothrombosis ischemia was used in the experiment. Brains of animals from the experimental group were removed and placed in liquid N(2) for 60 min after the green laser exposure, the control group brains, exposed to the photosensitive dye Rose Bengal (RB), were placed in liquid N(2) for 80 min after RB application, naive control brains were also briefly stored in liquid N(2). Spectroscopy of electron paramagnetic (spin) resonance was used to directly measure FR (hydroxyl (OH(.)) and nitroxyl (NO(.)). Compared to naive controls, both the ischemia and RB groups had significantly higher levels of OH(.), however, there were no differences between them. Comparison of hemispheres, i.e., with and without ischemia, in the experimental group did not show any significant difference in OH(.). NO(.) were elevated in the ischemia and RB groups compare to naive controls. Higher levels of NO(.) were found in hemispheres with ischemia compared to unexposed hemispheres. Increases in OH(.) were probably associated with the action of RB itself in this model of ischemia. Increases in NO(.) were closely related to the pathogenesis of photothrombotic ischemia and could be related to the activity of nitric oxide synthases.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Physiology
Radical
Ischemia
Brain Ischemia
Nitric oxide
Brain ischemia
Lesion
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
Rose bengal
Animals
Rats, Wistar
Cerebral Cortex
Hydroxyl Radical
Electron Spin Resonance Spectroscopy
General Medicine
medicine.disease
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Cerebral cortex
Nitrogen Oxides
Hydroxyl radical
medicine.symptom
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18029973 and 08628408
- Database :
- OpenAIRE
- Journal :
- Physiological Research
- Accession number :
- edsair.doi.dedup.....dcc8e93b5fac450332048c4a2f6ac319