Identification of Olfactory Genes in Monochamus saltuarius and Effects of Bursaphelenchus xylophilus Infestation on Their Expression

The pine wood nematode (PWN) Bursaphelenchus xylophilus has caused disastrous losses of pine forests in many countries, and the success of PWN depends strongly on interactions with its insect vectors. Monochamus saltuarius is a newly recorded vector in Northeast China. Feeding (i.e., immature) and egg-laying (i.e., mature) Monochamus spp. target different host plants, and olfactory cues play important roles regarding host choice. Whether infestation with PWN affects olfactory mechanisms in M. saltuarius related to feeding and oviposition is of interest as this may affect the spread of nematodes to new healthy hosts. However, little is known about molecular mechanisms of the olfactory system of M. saltuarius. We identified chemosensory-related genes in adult M. saltuarius and examined the influence of B. xylophilus on the respective expression patterns. Fifty-three odorant-binding proteins (OBPs), 15 chemosensory proteins, 15 olfactory receptors (ORs), 10 gustatory receptors, 22 ionotropic receptors (IRs), and two sensory neuron membrane proteins were identified, and sex bias among non-infested beetles was mainly found with respect to expression of OBPs. Interestingly, OBPs and ORs were markedly down-regulated in male M. saltuarius infested with B. xylophilus, which may reduce olfactory sensitivity of male M. saltuarius and affect the spreading of B. xylophilus to new hosts. Our results will help understand the interactions between B. xylophilus and M. saltuarius, which may lead to the identification of new control targets in the olfactory system of M. saltuarius.