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Differential effects of raloxifene and tamoxifen on the expression of estrogen receptors and antigen Ki-67 in human endometrial adenocarcinoma cell line
- Source :
- Scopus-Elsevier, ResearcherID
-
Abstract
- Tamoxifen and raloxifene are widely used in clinical practice. It has been found that tamoxifen treatment increases the risk of development of endometrial cancer. The effects of tamoxifen and raloxifene on endometrium might be caused by different estrogen receptor expression. The aim of the present study was immunohistochemical evaluation of the effects of tamoxifen and raloxifene on estrogen receptors, and Ki-67 antigen expression in the human endometrial adenocarcinoma Ishikawa cell line. Tamoxifen in concentrations of 10 microM and 20 microM increased ERalpha expression without any effect on ERbeta. All used concentrations of tamoxifen and raloxifene (0.1 nM, 1 nM, 10 nM, 1 micro M, 10 microM and 20 microM) had no effect on expression of ERbeta. Tamoxifen, but not raloxifene, increased Ki-67 antigen expression in the Ishikawa cell line. Tamoxifen, in contrast to raloxifene, increased proliferation of endometrial adenocarcinoma cells as well as exerted the shift of ERalpha/ERbeta ratio. Thus, it could be responsible for increased carcinogenic effect during tamoxifen treatment.
- Subjects :
- Cancer Research
medicine.medical_specialty
Antineoplastic Agents, Hormonal
medicine.drug_class
Estrogen receptor
Adenocarcinoma
Biology
Endometrium
Cell Line, Tumor
Internal medicine
medicine
Humans
Raloxifene
skin and connective tissue diseases
Endometrial cancer
Estrogen Antagonists
General Medicine
Antiestrogen
medicine.disease
Immunohistochemistry
Endometrial Neoplasms
Gene Expression Regulation, Neoplastic
Tamoxifen
Ki-67 Antigen
medicine.anatomical_structure
Endocrinology
Receptors, Estrogen
Oncology
Estrogen
Raloxifene Hydrochloride
Female
Cell Division
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier, ResearcherID
- Accession number :
- edsair.doi.dedup.....dc9e1062b17c9d87095b12d9b0cb74e7