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LncRNA CRNDE promotes the progression and angiogenesis of pancreatic cancer via miR-451a/CDKN2D axis
- Source :
- Translational Oncology, Translational Oncology, Vol 14, Iss 7, Pp 101088-(2021)
- Publication Year :
- 2021
- Publisher :
- Neoplasia Press, 2021.
-
Abstract
- Highlights • CRNDE was up-regulated in pancreatic cancer. • CRNDE promoted the progression and angiogenesis of pancreatic cancer. • CRNDE functioned as a sponge for miR-451a in pancreatic cancer cells. • MiR-451a directly interacted with CDKN2D and regulated CDKN2D expression. • CRNDE regulated pancreatic cancer progression via miR-451a/CDKN2D axis.<br />Background The lncRNA colorectal neoplasia differentially expressed (lncRNA CRNDE) has been reported to play a pivotal role in various cancers. However, the expression and function of CRNDE in pancreatic cancer remain unclear. The objective of this study was to investigate the effects of CRNDE on pancreatic cancer and the underlying mechanisms. Methods The expression of CRNDE in pancreatic cancer tissues and cell lines was determined by RT-qPCR. Proliferation and angiogenesis were detected by MTT, colony formation, transwell and tube formation assays in vitro and in vivo. ELISA assay was used to detect the secretion of VEGFA. IHC was performed to test the expression levels of Ki67 and CD31. The binding sites between CRNDE, CDKN2D and miR-451a were predicted by bioinformatics analysis. Dual luciferase reporter and RNA immunoprecipitation assays were conducted to confirm the interaction with each other. Results The results showed that CRNDE was significantly up-regulated in pancreatic cancer tissues as well as cell lines. CRNDE overexpression promoted the progression and angiogenesis of pancreatic cancer cells in vitro and in vivo. Moreover, we identified that CRNDE functioned as a sponge for miR-451a and CRNDE overexpression inhibited the expression of miR-451a. Furthermore, we confirmed that miR-451a directly interacted with CDKN2D and negatively regulated CDKN2D expression. In addition, CRNDE was found to positively regulate CDKN2D expression and mediate pancreatic cancer cell proliferation and angiogenesis through miR-451a/CDKN2D axis. Conclusion CRNDE modulates cell proliferation and angiogenesis via miR-451a/CDKN2D axis in pancreatic cancer, which provides a potential therapeutic target for pancreatic cancer treatment.<br />Graphical abstract Image, graphical abstract
- Subjects :
- 0301 basic medicine
CD31
Cancer Research
Angiogenesis
Proliferation
Long non-coding RNAs lncRNAs, LncRNA colorectal neoplasia differentially expressed lncRNA CRNDE
Biology
03 medical and health sciences
0302 clinical medicine
Enzyme linked immunosorbent assay ELISA, Immunohistochemistry IHC
CRNDE
Pancreatic cancer
medicine
CDKN2D
RC254-282
Original Research
Tube formation
Cell growth
Mir-451a
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Vascular endothelial growth factor A
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer research
Immunohistochemistry
MicroRNAs miRNAs, RNA immunoprecipitation RIP
Subjects
Details
- Language :
- English
- ISSN :
- 19365233
- Volume :
- 14
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Translational Oncology
- Accession number :
- edsair.doi.dedup.....dc96eab1166115eb418c6fb3d07ac0e5