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Phosphoketolase pathway engineering for carbon-efficient biocatalysis

Authors :
Emily F. Freed
Calvin A. Henard
Michael T. Guarnieri
Source :
Current opinion in biotechnology. 36
Publication Year :
2015

Abstract

Recent advances in metabolic engineering have facilitated the development of microbial biocatalysts capable of producing an array of bio-products, ranging from fuels to drug molecules. These bio-products are commonly generated through an acetyl-CoA intermediate, which serves as a key precursor in the biological conversion of carbon substrates. Conventional biocatalytic upgrading strategies proceeding through this route are limited by low carbon efficiencies, in large part due to carbon losses associated with pyruvate decarboxylation to acetyl-CoA. Bypass of pyruvate decarboxylation offers a means to dramatically enhance carbon yields and, in turn, bioprocess economics. Herein, we discuss recent advances and prospects for employing the phosphoketolase pathway for direct biosynthesis of acetyl-CoA from carbon substrates, and phosphoketolase-based metabolic engineering strategies for carbon efficient biocatalysis.

Details

ISSN :
18790429
Volume :
36
Database :
OpenAIRE
Journal :
Current opinion in biotechnology
Accession number :
edsair.doi.dedup.....dc79f340dce110b4be77c5daea35ce24