Back to Search
Start Over
In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates produced by Pseudomonas putida Bet001
- Publication Year :
- 2017
- Publisher :
- Taylor & Francis, 2017.
-
Abstract
- In vivo and in vitro depolymerizations of intracellular medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 grown on lauric acid was studied. Both processes were studied under optimum conditions for mcl-PHA depolymerization viz. 0.2 M Tris-HCl buffer, pH 9, ionic strength (I) = 0.2 M at 30 °C. For in vitro depolymerization studies, cell-free system was obtained from lysing bacterial cells suspension by ultrasonication at optimum conditions (frequency 37 kHz, 30% of power output, °C for 120 minutes). The comparison between in vivo and in vitro depolymerizations of intracellular mcl-PHA was made. In vitro depolymerization showed lower depolymerization rate but higher yield compared to in vivo depolymerization. The monomer liberation rate reflected the mol percentage distribution of the initial polymer subunit composition, and the resulting direct individual products of depolymerization were identical for both in vivo and in vitro processes. It points to exo-type reaction for both processes, and potential biological route to chiral molecules.
- Subjects :
- 0301 basic medicine
Lysis
030106 microbiology
Biochemistry
Polyhydroxyalkanoates
Polymerization
03 medical and health sciences
chemistry.chemical_compound
In vivo
Tromethamine
biology
Pseudomonas putida
Depolymerization
Chemistry
Osmolar Concentration
Temperature
Lauric Acids
General Medicine
biology.organism_classification
Lauric acid
In vitro
030104 developmental biology
Ionic strength
Biophysics
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....dc751da6dc772f0f92773d24c9fefa6b
- Full Text :
- https://doi.org/10.6084/m9.figshare.5132893