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Spare interactions of highly potent [Arg(14),Lys(15)]nociceptin for cooperative induction of ORL1 receptor activation
Spare interactions of highly potent [Arg(14),Lys(15)]nociceptin for cooperative induction of ORL1 receptor activation
- Source :
- Bioorganicmedicinal chemistry. 17(23)
- Publication Year :
- 2009
-
Abstract
- [Arg(14),Lys(15)]Nociceptin is a very potent for ORL1 receptor, showing a few times stronger binding activity and much more enhanced biological activity than endogenous nociceptin. This synergistic outcome has been suggested to be due to the interaction with the receptor aromatic and/or acidic amino acid residues crucial to receptor activation. In order to identify such receptor residues in the second ORL1 extracellular loop, we prepared a series of recombinant mutant receptors. The mutant receptor Gln205Ala was found to be as active as wild-type ORL1 for both nociceptin and [Arg(14),Lys(15)]nociceptin. In contrast, Asp206Ala and Tyr207Ala exhibited considerably reduced activity for [Arg(14),Lys(15)]nociceptin, exhibiting no synergistic activity enhancement. These results suggest that Asp206 and Tyr207 are directly involved in the interaction with nociceptin-[Arg(14),Lys(15)]. Trp208Ala was found to bind strongly both nociceptin and [Arg(14),Lys(15)]nociceptin, although it elicited no biological activity. All these results indicate that the consecutive amino acid residues Asp206, Tyr207, and Trp208 are critical to the activation of the ORL1 receptor, but not to nociceptin-binding.
- Subjects :
- Agonist
Arginine
Stereochemistry
medicine.drug_class
Clinical Biochemistry
Molecular Sequence Data
Pharmaceutical Science
Biochemistry
Binding, Competitive
Nociceptin Receptor
Drug Discovery
Tachykinin receptor 1
medicine
Amino Acid Sequence
Binding site
Receptor
Molecular Biology
Peptide sequence
Chemistry
Lysine
Organic Chemistry
Biological activity
Nociceptin receptor
Kinetics
Opioid Peptides
Receptors, Opioid
Mutagenesis, Site-Directed
Molecular Medicine
Subjects
Details
- ISSN :
- 14643391
- Volume :
- 17
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Bioorganicmedicinal chemistry
- Accession number :
- edsair.doi.dedup.....dc62ba760726be0a391085bbceb9aac2