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Biosynthesis and Cytotoxic Properties of Ag, Au and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 17, International Journal of Molecular Sciences, Vol 22, Iss 9305, p 9305 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), and bimetallic Ag/Au nanoparticles using the callus extract of Lithospermum&nbsp<br />erythrorhizon as a reducing and capping agent. The biosynthesized nanoparticles were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). Our results showed the formation of crystalline metal nanostructures of both spherical and non-spherical shape. Energy dispersive X-ray (EDX) spectroscopy showed the characteristic peaks in the silver and gold regions, confirming the presence of the corresponding elements in the monometallic particles and both elements in the bimetallic particles. Fourier-transform infrared (FTIR) spectroscopy affirmed the role of polysaccharides and polyphenols of the L.&nbsp<br />erythrorhizon extract as the major reducing and capping agents for metal ions. In addition, our results showed that the polysaccharide sample and the fraction containing secondary metabolites isolated from L.&nbsp<br />erythrorhizon were both able to produce large amounts of metallic nanoparticles. The biosynthesized nanoparticles demonstrated cytotoxicity against mouse neuroblastoma and embryonic fibroblast cells, which was considerably higher for Ag nanoparticles and for bimetallic Ag/Au nanoparticles containing a higher molar ratio of silver. However, fibroblast migration was not significantly affected by any of the nanoparticles tested. The obtained results provide a new example of the safe biological production of metallic nanoparticles, but further study is required to uncover the mechanism of their toxicity so that the biomedical potency can be assessed.
- Subjects :
- biomedical applications
silver nanoparticles
Silver
QH301-705.5
Metal ions in aqueous solution
Nanoparticle
Metal Nanoparticles
Antineoplastic Agents
Apoptosis
Catalysis
Silver nanoparticle
Article
Fibroblast migration
Inorganic Chemistry
Mice
Neuroblastoma
Ag/Au nanoparticles
Animals
Physical and Theoretical Chemistry
Fourier transform infrared spectroscopy
Biology (General)
Molecular Biology
Bimetallic strip
QD1-999
Spectroscopy
Cells, Cultured
biology
Chemistry
Plant Extracts
plant cell culture
Lithospermum
green synthesis
Organic Chemistry
in vitro
General Medicine
Lithospermum erythrorhizon
biology.organism_classification
Computer Science Applications
anticancer drugs
Colloidal gold
gold nanoparticles
NIH 3T3 Cells
Gold
Nuclear chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....dc48bcf1cb59ba04d019db7920a2e2c2
- Full Text :
- https://doi.org/10.3390/ijms22179305