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The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes
- Source :
- Nucleic Acids Research
- Publication Year :
- 2018
-
Abstract
- In Arabidopsis, the circadian clock central oscillator genes are important cellular components to generate and maintain circadian rhythms. There is a negative feedback loop between the morning expressed CCA1 (CIRCADIAN CLOCK ASSOCIATED 1)/LHY (LATE ELONGATED HYPOCOTYL) and evening expressed TOC1 (TIMING OF CAB EXPRESSION 1). CCA1 and LHY negatively regulate the expression of TOC1, while TOC1 also binds to the promoters of CCA1 and LHY to repress their expression. Recent studies indicate that histone modifications play an important role in the regulation of the central oscillators. However, the regulatory relationship between histone modifications and the circadian clock genes remains largely unclear. In this study, we found that the Lysine-Specific Demethylase 1 (LSD1)-like histone demethylases, LDL1 and LDL2, can interact with CCA1/LHY to repress the expression of TOC1. ChIP-Seq analysis indicated that LDL1 targets a subset of genes involved in the circadian rhythm regulated by CCA1. Furthermore, LDL1 and LDL2 interact with the histone deacetylase HDA6 and co-regulate TOC1 by histone demetylation and deacetylaion. These results provide new insight into the molecular mechanism of how the circadian clock central oscillator genes are regulated through histone modifications.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Circadian clock
TOC1
Arabidopsis
Biology
01 natural sciences
Histone Deacetylases
03 medical and health sciences
Gene Expression Regulation, Plant
Circadian Clocks
Genetics
Circadian rhythm
Regulation of gene expression
Histone Demethylases
Arabidopsis Proteins
Gene Expression Profiling
Gene regulation, Chromatin and Epigenetics
Circadian Clock Associated 1
Cell biology
DNA-Binding Proteins
Histone Code
030104 developmental biology
Histone
Multiprotein Complexes
biology.protein
Demethylase
010606 plant biology & botany
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 13624962
- Volume :
- 46
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....dc3e7ee79c9d86eebca25e15e1515122