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Localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling
- Source :
- E-Prints Complutense. Archivo Institucional de la UCM, instname, E-Prints Complutense: Archivo Institucional de la UCM, Universidad Complutense de Madrid
- Publication Year :
- 2014
- Publisher :
- Elsevier France-Editions Scientifiques Médicales Elsevier, 2014.
-
Abstract
- The presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. Furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. With the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections of isolated and intact adaxial leaf cuticles of Eucalyptus globulus, Populus × canescens and Pyrus communis by means of enzyme gold-labelling (Au-cellulase, EC 3.2.1.4, and -pectinase, EC 3.2.1.15) and transmission electron microscopy (TEM). The structure of the interface between the cuticle and the cell wall underneath was observed to influence the process of enzymatic isolation of leaf cuticles. Cellulose and pectins were detected for the first time in enzymatically isolated cuticles, sometimes appearing closely underneath the epicuticular wax layer. The location and presence of polysaccharides in intact and isolated leaf cuticles may have multiple implications, such as when estimating the bi-directional transport of substances between plant organs and the surrounding environment, or when interpreting organ ontogeny. The results are discussed within a plant ontological and ecophysiological context.
- Subjects :
- Physiology
Cuticle
Context (language use)
Plant Science
Biology
Polysaccharide
Epicuticular wax
Plant Epidermis
Cell wall
Pyrus
chemistry.chemical_compound
Microscopy, Electron, Transmission
Cell Wall
Polysaccharides
Botany
Genetics
Cellulose
Pectinase
chemistry.chemical_classification
Eucalyptus
fungi
Termodinámica
biology.organism_classification
Enzymes
Plant Leaves
Populus
chemistry
Pectins
Gold
Pyrus communis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- E-Prints Complutense. Archivo Institucional de la UCM, instname, E-Prints Complutense: Archivo Institucional de la UCM, Universidad Complutense de Madrid
- Accession number :
- edsair.doi.dedup.....dc2e9ec3965cf4ea24f38bcb92c6af65