Purification and crystallization of Phd, the antitoxin of the phd/doc operon

Authors :: Yann G.-J. Sterckx
Guy Vandenbussche
Remy Loris
Abel Garcia-Pino
Structural Biology Brussels
Ultrastructure
Department of Bio-engineering Sciences
Publication Year :: 2010
Publisher :: International Union of Crystallography, 2010.
Abstract: The antitoxin Phd from the phd/doc module of bacteriophage P1 was crystallized in two distinct crystal forms. Crystals of His-tagged Phd contain a C-terminally truncated version of the protein and diffract to 2.20 A resolution. Crystals of untagged Phd purified from the Phd-Doc complex diffract to 2.25 A resolution. These crystals are partially merohedrally twinned and contain the full-length version of the protein.

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