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Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall
- Source :
- Arquivos Brasileiros de Cardiologia, Volume: 74, Issue: 2, Pages: 124-128, Published: FEB 2000, Arquivos Brasileiros de Cardiologia, Vol 74, Iss 2, Pp 124-128 (2000), Arquivos Brasileiros de Cardiologia v.74 n.2 2000, Arquivos Brasileiros de Cardiologia, Sociedade Brasileira de Cardiologia (SBC), instacron:SBC
- Publication Year :
- 2000
- Publisher :
- Sociedade Brasileira de Cardiologia - SBC, 2000.
-
Abstract
- OBJECTIVE: The intracellular Gram-negative bacterium Chlamydia pneumoniae has been associated with atherosclerosis. The presence of Chlamydia pneumoniae has been investigated in fragments of the arterial wall with a technique for DNA identification. METHODS: Arterial fragments obtained from vascular surgical procedures in 58 patients were analyzed. From these patients, 39 were males and the mean age was 65±6 years. The polymerase chain reaction was used to identify the bacterial DNA with a pair of primers that codify the major outer membrane protein (MOMP) of Chlamydia pneumoniae. The amplified product was visualized by electrophoresis in the 2% agarose gel stained with ethidium bromide, and it was considered positive when migrating in the band of molecular weight of the positive controls. RESULTS: Seven (12%) out of the 58 patients showed positive results for Chlamydia pneumoniae. CONCLUSION: DNA from Chlamydia pneumoniae was identified in the arterial wall of a substantial number of patients with atherosclerosis. This association, which has already been described in other countries, corroborates the evidence favoring a role played by Chlamydia pneumoniae in atherogenesis.
- Subjects :
- DNA, Bacterial
Male
lcsh:Diseases of the circulatory (Cardiovascular) system
Arteriosclerosis
polymerase chain reaction
urologic and male genital diseases
law.invention
Microbiology
chemistry.chemical_compound
Risk Factors
law
Chlamydia pneumoniae
medicine
Humans
Polymerase chain reaction
Aged
DNA Primers
Chlamydia
biology
business.industry
Arteries
Chlamydia Infections
Middle Aged
medicine.disease
biology.organism_classification
female genital diseases and pregnancy complications
chemistry
lcsh:RC666-701
Female
atherosclerosis
Cardiology and Cardiovascular Medicine
Bacterial outer membrane
Ethidium bromide
business
Intracellular
DNA
Bacteria
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Arquivos Brasileiros de Cardiologia, Volume: 74, Issue: 2, Pages: 124-128, Published: FEB 2000, Arquivos Brasileiros de Cardiologia, Vol 74, Iss 2, Pp 124-128 (2000), Arquivos Brasileiros de Cardiologia v.74 n.2 2000, Arquivos Brasileiros de Cardiologia, Sociedade Brasileira de Cardiologia (SBC), instacron:SBC
- Accession number :
- edsair.doi.dedup.....dc195182c5b1c30506de2c5b3a00c2b1