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On-chip purification and detection of hepatitis C virus RNA from human plasma
- Source :
- Biophysical chemistry, 208 (2016): 54–61. doi:10.1016/j.bpc.2015.06.005, info:cnr-pdr/source/autori:Vaghi V.; Potrich C.; Pasquardini L.; Lunelli L.; Vanzetti L.; Ebranati E.; Lai A.; Zehender G.; Mombello D.; Cocuzza M.; Pirri C.F.; Pederzolli C./titolo:On-chip purification and detection of hepatitis C virus RNA from human plasma/doi:10.1016%2Fj.bpc.2015.06.005/rivista:Biophysical chemistry (Print)/anno:2016/pagina_da:54/pagina_a:61/intervallo_pagine:54–61/volume:208
- Publication Year :
- 2016
- Publisher :
- ELSEVIER SCIENCE BV, 2016.
-
Abstract
- Hepatitis C virus (HCV) is one of the main causes of chronic liver disease worldwide. The diagnosis and monitoring of HCV infection is a crucial need in the clinical management. The conventional diagnostic technologies are challenged when trying to address molecular diagnostics, especially because they require a complex and time-consuming sample preparation phase. Here, a new concept based on surface functionalization was applied to viral RNA purification: first of all polydimethylsiloxane (PDMS) flat surfaces were modified to hold RNA adsorption. After a careful chemical and morphological analysis of the modified surfaces, the functionalization protocols giving the best RNA adsorbing surfaces were applied to PDMS microdevices. The functionalized microdevices were then used for RNA purification from HCV infected human plasma samples. RNA purification and RT were successfully performed in the same microdevice chamber, saving time of analysis, reagents, and labor. The PCR protocol for HCV cDNA amplification was also implemented in the microdevice, demonstrating that the entire process of HCV analysis, from plasma to molecular readout, could be performed on-chip. Not only HCV but also other microdevice-based viral RNA detection could therefore result in a successful Point-of-Care (POC) diagnostics for resource-limited settings.
- Subjects :
- 0301 basic medicine
Surface Properties
Hepatitis C virus
Biophysics
PDMS microdevice
Hepacivirus
medicine.disease_cause
Polymerase Chain Reaction
Biochemistry
03 medical and health sciences
On-chip RT-PCR
Lab-On-A-Chip Devices
Complementary DNA
Hepatitis C virus RNA
medicine
On-chip viral RNA purification
Humans
Dimethylpolysiloxanes
Chemistry
Organic Chemistry
RNA
Molecular diagnostics
Virology
030104 developmental biology
Real-time polymerase chain reaction
Human plasma
RNA, Viral
Adsorption
RNA extraction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biophysical chemistry, 208 (2016): 54–61. doi:10.1016/j.bpc.2015.06.005, info:cnr-pdr/source/autori:Vaghi V.; Potrich C.; Pasquardini L.; Lunelli L.; Vanzetti L.; Ebranati E.; Lai A.; Zehender G.; Mombello D.; Cocuzza M.; Pirri C.F.; Pederzolli C./titolo:On-chip purification and detection of hepatitis C virus RNA from human plasma/doi:10.1016%2Fj.bpc.2015.06.005/rivista:Biophysical chemistry (Print)/anno:2016/pagina_da:54/pagina_a:61/intervallo_pagine:54–61/volume:208
- Accession number :
- edsair.doi.dedup.....dc0f31e3b06385ab991292e04a2daa3e