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Effects of in Utero PFOS Exposure on Transcriptome, Lipidome, and Function of Mouse Testis
- Source :
- Environmental Science and Technology, Environmental Science and Technology, American Chemical Society, 2017, 51 (15), pp.8782-8794. ⟨10.1021/acs.est.7b02102⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Transcriptomic and LC-MS/MS-based targeted lipidomic analyses were conducted to identify the effects of in utero PFOS exposure on neonatal testes and its relation to testicular dysfunction in adult offspring. Pregnant mice were orally administered 0.3 and 3 μg PFOS/g body weight until term. Neonatal testes (P1) were collected for the detection of PFOS, and were subjected to omics study. Integrated pathway analyses using DAVID, KEGG, and IPA underlined the effects of PFOS exposure on lipid metabolism, oxidative stress and cell junction signaling in testes. LC-MS/MS analysis showed that the levels of adrenic acid and docosahexaenoic acid (DHA) in testes were significantly reduced in the PFOS treatment groups. A significant linear decreasing trend in eicosapentaenoic acid and DHA with PFOS concentrations was observed. Moreover, LOX-mediated 5hydroxyeicosatetraenoic acids (HETE) and 15-HETE from arachidonic acid in the testes were significantly elevated and a linear increasing trend of 15-HETE concentrations was detected with doses of PFOS. The perturbations of lipid mediators suggested that PFOS has potential negative impacts on testicular functions. Postnatal analysis of male offspring at P63 showed significant reductions in serum testosterone and epididymal sperm count. This study sheds light into the as yet unrevealed action of PFOS on lipid mediators in affecting testicular functions.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
[SDV]Life Sciences [q-bio]
Biology
medicine.disease_cause
Transcriptome
Mice
03 medical and health sciences
chemistry.chemical_compound
Pregnancy
Tandem Mass Spectrometry
Internal medicine
Hydroxyeicosatetraenoic Acids
Testis
medicine
Animals
Environmental Chemistry
Fluorocarbons
Sperm Count
Lipid metabolism
General Chemistry
Lipidome
Eicosapentaenoic acid
030104 developmental biology
Endocrinology
Alkanesulfonic Acids
chemistry
Docosahexaenoic acid
In utero
Female
Arachidonic acid
lipids (amino acids, peptides, and proteins)
Water Pollutants, Chemical
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 0013936X and 15205851
- Database :
- OpenAIRE
- Journal :
- Environmental Science and Technology, Environmental Science and Technology, American Chemical Society, 2017, 51 (15), pp.8782-8794. ⟨10.1021/acs.est.7b02102⟩
- Accession number :
- edsair.doi.dedup.....dc0dfdd65a9eb911bf237c7bbfd55b72