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Reduction of paraoxonase-1 activity may contribute the qualitative impairment of HDL particles in patients with type 2 diabetes
- Source :
- Diabetes research and clinical practice. 99(1)
- Publication Year :
- 2012
-
Abstract
- Aims Cholesterol efflux with high-density lipoprotein (HDL) particles has an important role in the first step of reverse cholesterol transport (RCT). However, HDL function in type 2 diabetes has not been well investigated thoroughly. We measured cholesterol efflux in 36 patients with type 2 diabetes compared with 9 controls without diabetes. Methods The HDL fraction was separated with polyacrylamide gel and recovered using the protein recovery system. Concentration adjusted HDL fraction was used to determine HDL-mediated cholesterol efflux (Efflux-hdl) from THP-1 derived macrophages. We measured paraoxonase-1 (PON 1) activity to determine antioxidation capacity, serum amyloid A protein (SAA) to determine inflammatory response, and carboxymethyl-lysin (CML) to determine antiglucoxidative capacity. Results Efflux-hdl demonstrated no correlation with plasma apoprotein A-1 (ApoA-I) or HDL-cholesterol in patients with diabetes. PON1 activity in the patients’ HDL fraction was positively correlated with Efflux-hdl (r = 0.39, p = 0.02), and showed a negative tendency with HbA1c levels (r = −0.28, p = 0.10). SAA and CML levels did not demonstrate correlation with Efflux-hdl in patients with diabetes. Conclusion We confirmed the functional changes in HDL particles in the patients. Efflux-hdl from macrophages was reduced depending upon the decrease in PON1 activity, which was inversely related to HbA1c levels.
- Subjects :
- Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Type 2 diabetes
Cell Line
chemistry.chemical_compound
Endocrinology
Internal medicine
Diabetes mellitus
Internal Medicine
medicine
Humans
Serum Amyloid A Protein
Aged
Glycated Hemoglobin
biology
Apolipoprotein A-I
business.industry
Cholesterol
Aryldialkylphosphatase
Lysine
Macrophages
Reverse cholesterol transport
Cholesterol, HDL
Paraoxonase
nutritional and metabolic diseases
Biological Transport
General Medicine
Middle Aged
medicine.disease
Atherosclerosis
PON1
chemistry
Diabetes Mellitus, Type 2
Hyperglycemia
biology.protein
lipids (amino acids, peptides, and proteins)
Female
business
Lipoproteins, HDL
Biomarkers
Lipoprotein
Subjects
Details
- ISSN :
- 18728227
- Volume :
- 99
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Diabetes research and clinical practice
- Accession number :
- edsair.doi.dedup.....dc0c88bb2b5af248fee68a0eb5532fa3