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Reduction of paraoxonase-1 activity may contribute the qualitative impairment of HDL particles in patients with type 2 diabetes

Authors :
Hiroshi Murakami
Jutaro Tanabe
Naoki Tamasawa
Koki Matsumura
Maki Yamashita
Kota Matsuki
Jun Matsui
Toshihiro Suda
Source :
Diabetes research and clinical practice. 99(1)
Publication Year :
2012

Abstract

Aims Cholesterol efflux with high-density lipoprotein (HDL) particles has an important role in the first step of reverse cholesterol transport (RCT). However, HDL function in type 2 diabetes has not been well investigated thoroughly. We measured cholesterol efflux in 36 patients with type 2 diabetes compared with 9 controls without diabetes. Methods The HDL fraction was separated with polyacrylamide gel and recovered using the protein recovery system. Concentration adjusted HDL fraction was used to determine HDL-mediated cholesterol efflux (Efflux-hdl) from THP-1 derived macrophages. We measured paraoxonase-1 (PON 1) activity to determine antioxidation capacity, serum amyloid A protein (SAA) to determine inflammatory response, and carboxymethyl-lysin (CML) to determine antiglucoxidative capacity. Results Efflux-hdl demonstrated no correlation with plasma apoprotein A-1 (ApoA-I) or HDL-cholesterol in patients with diabetes. PON1 activity in the patients’ HDL fraction was positively correlated with Efflux-hdl (r = 0.39, p = 0.02), and showed a negative tendency with HbA1c levels (r = −0.28, p = 0.10). SAA and CML levels did not demonstrate correlation with Efflux-hdl in patients with diabetes. Conclusion We confirmed the functional changes in HDL particles in the patients. Efflux-hdl from macrophages was reduced depending upon the decrease in PON1 activity, which was inversely related to HbA1c levels.

Details

ISSN :
18728227
Volume :
99
Issue :
1
Database :
OpenAIRE
Journal :
Diabetes research and clinical practice
Accession number :
edsair.doi.dedup.....dc0c88bb2b5af248fee68a0eb5532fa3