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Zinc treatment modulates hematological and morphological changes in rat erythrocytes following arsenic exposure
- Source :
- Toxicology and Industrial Health. 35:593-603
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- The aim of this study was to understand the effects of zinc supplementation on antioxidant defense systems, hematological indices, and erythrocyte morphology in conditions of chronic arsenic toxicity. Male Wistar rats were segregated into four groups: control, arsenic treated, zinc supplemented, and arsenic + zinc treated. The animals in the arsenic-treated group were given arsenic orally in drinking water in the form of sodium arsenite at a dose level of 100 mg L−1, and zinc was administered to zinc-treated animals in the form of zinc sulfate orally in drinking water at a dose level of 227 mg L−1. The animals were subjected to different treatments for a period of 12 weeks, and various investigations were undertaken that included serum zinc content, activity of antioxidant enzymes, and hematological indices. Further, scanning electron microscopic (SEM) studies were performed to assess morphological changes in erythrocytes. Arsenic treatment significantly reduced serum zinc concentrations, which, however, were restored to near-normal levels upon zinc supplementation. The activities of enzymes involved in antioxidant defense systems were altered in the erythrocyte lysates of arsenic-treated rats, which interestingly revealed a significant improvement upon simultaneous zinc supplementation. A significant reduction in the counts of total leukocytes, neutrophils, and lymphocytes was observed following arsenic intoxication, which came back to near control levels following zinc supplementation. Also, protective effects of zinc were evident from SEM that revealed maintenance of topographical appearances of erythrocytes in conditions of arsenic toxicity. Thus, this study clearly shows the protection afforded by zinc on erythrocytes during arsenic-induced toxicity.
- Subjects :
- Male
medicine.medical_specialty
Erythrocytes
Antioxidant
Sodium arsenite
Health, Toxicology and Mutagenesis
medicine.medical_treatment
chemistry.chemical_element
Zinc
010501 environmental sciences
Toxicology
01 natural sciences
Arsenic
Hemoglobins
Leukocyte Count
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
medicine
Animals
Rats, Wistar
ARSENIC EXPOSURE
Glutathione Transferase
030304 developmental biology
0105 earth and related environmental sciences
chemistry.chemical_classification
0303 health sciences
Arsenic toxicity
Public Health, Environmental and Occupational Health
Catalase
Glutathione
Rats
Enzyme
Endocrinology
chemistry
Toxicity
Microscopy, Electron, Scanning
Lipid Peroxidation
Subjects
Details
- ISSN :
- 14770393 and 07482337
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Toxicology and Industrial Health
- Accession number :
- edsair.doi.dedup.....dbfb1cc9a05aa7efa84f2ccda77d943f