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The signaling peptide-encoding genes CLE16, CLE17 and CLE27 are dispensable for Arabidopsis shoot apical meristem activity
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 8, p e0202595 (2018)
- Publication Year :
- 2018
-
Abstract
- The shoot apical meristem produces all of the leaves, stems and flowers of a flowering plant from a reservoir of stem cells at its growing tip. In Arabidopsis, the small polypeptide signaling molecule CLAVATA3 (CLV3), a member of the CLV3/EMBRYO SURROUNDING REGION-RELATED (CLE) gene family, is a key component of a negative feedback loop that maintains stem cell activity in shoot and floral meristems throughout development. Because in some plant species multiple CLE genes are involved in regulating shoot apical meristem activity, we tested the hypothesis that CLE genes other than CLV3 might function in stem cell homeostasis in Arabidopsis. We identified three Arabidopsis CLE genes expressed in the post-embryonic shoot apical meristem, generated loss-of-function alleles using genome editing, and analyzed the meristem phenotypes of the resulting mutant plants. We found that null mutations in CLE16, CLE17 or CLE27 affected neither vegetative nor reproductive shoot meristem activity under normal growth conditions, although CLE27 appears to slightly prolong vegetative growth. Our results indicate that the CLE16, CLE17 and CLE27 genes have largely redundant roles in the Arabidopsis shoot apical meristem and/or regulate meristem activity only under specific environmental conditions.
- Subjects :
- 0301 basic medicine
Leaves
Mutant
Arabidopsis
lcsh:Medicine
Floral Meristem
Plant Science
Meristems
Gene Expression Regulation, Plant
lcsh:Science
Flowering Plants
Multidisciplinary
biology
Plant Anatomy
food and beverages
Eukaryota
Embryo
Plants
Phenotype
Cell biology
Experimental Organism Systems
Plant Physiology
Shoot
Plant Shoots
Research Article
Arabidopsis Thaliana
Meristem
Flowers
Brassica
Protein Sorting Signals
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Plant and Algal Models
Gene family
Amino Acid Sequence
Gene
Alleles
Homeodomain Proteins
Arabidopsis Proteins
lcsh:R
fungi
Organisms
Biology and Life Sciences
Stem Anatomy
biology.organism_classification
Plant Leaves
030104 developmental biology
Shoot Apical Meristem
Seedlings
Mutation
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....dbf62ac86fecd8062b2a6454f888e3f2