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Storage protein profiles in Spanish and runner market type peanuts and potential markers
- Source :
- BMC Plant Biology, Vol 6, Iss 1, p 24 (2006), BMC Plant Biology
- Publication Year :
- 2006
- Publisher :
- BMC, 2006.
-
Abstract
- Background Proteomic analysis has proven to be the most powerful method for describing plant species and lines, and for identification of proteins in complex mixtures. The strength of this method resides in high resolving power of two-dimensional electrophoresis (2-DE), coupled with highly sensitive mass spectrometry (MS), and sequence homology search. By using this method, we might find polymorphic markers to differentiate peanut subspecies. Results Total proteins extracted from seeds of 12 different genotypes of cultivated peanut (Arachis hypogaea L.), comprised of runner market (A. hypogaea ssp. hypogaea) and Spanish-bunch market type (A. hypogaea ssp. fastigiata), were separated by electrophoresis on both one- and two-dimensional SDS-PAGE gels. The protein profiles were similar on one-dimensional gels for all tested peanut genotypes. However, peanut genotype A13 lacked one major band with a molecular weight of about 35 kDa. There was one minor band with a molecular weight of 27 kDa that was present in all runner peanut genotypes and the Spanish-derivatives (GT-YY7, GT-YY20, and GT-YY79). The Spanish-derivatives have a runner-type peanut in their pedigrees. The 35 kDa protein in A13 and the 27 kDa protein in runner-type peanut genotypes were confirmed on the 2-D SDS-PAGE gels. Among more than 150 main protein spots on the 2-D gels, four protein spots that were individually marked as spots 1–4 showed polymorphic patterns between runner-type and Spanish-bunch peanuts. Spot 1 (ca. 22.5 kDa, pI 3.9) and spot 2 (ca. 23.5 kDa, pI 5.7) were observed in all Spanish-bunch genotypes, but were not found in runner types. In contrast, spot 3 (ca. 23 kDa, pI 6.6) and spot 4 (ca. 22 kDa, pI 6.8) were present in all runner peanut genotypes but not in Spanish-bunch genotypes. These four protein spots were sequenced. Based on the internal and N-terminal amino acid sequences, these proteins are isoforms (iso-Ara h3) of each other, are iso-allergens and may be modified by post-translational cleavage. Conclusion These results suggest that there may be an association between these polymorphic storage protein isoforms and peanut subspecies fastigiata (Spanish type) and hypogaea (runner type). The polymorphic protein peptides distinguished by 2-D PAGE could be used as markers for identification of runner and Spanish peanuts.
- Subjects :
- Proteomics
Arachis
Genotype
Molecular Sequence Data
Sequence alignment
Plant Science
Computational biology
Subspecies
Biology
Mass spectrometry
Species Specificity
Sequence Analysis, Protein
lcsh:Botany
Storage protein
Electrophoresis, Gel, Two-Dimensional
Amino Acid Sequence
Peptide sequence
Plant Proteins
chemistry.chemical_classification
food and beverages
Molecular biology
Arachis hypogaea
lcsh:QK1-989
chemistry
Genetic marker
Sequence Alignment
Biomarkers
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712229
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....dbe6f0f5c9f6d66300aa9da4bef12993