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Measurement of retinoids and beta-carotene 15,15'-dioxygenase activity in HR-1 hairless mouse skin with UV exposure
- Source :
- Journal of nutritional science and vitaminology. 49(1)
- Publication Year :
- 2003
-
Abstract
- We investigated the vitamin A status and beta-carotene 15,15'-dioxygenase activity in hairless mice with UV exposure to assess the regulation of vitamin A metabolism after UV irradiation. HR-1 hairless mice were irradiated with UV at 3 J/cm2 for 5 d. After UV irradiation, the mice were sacrificed and samples were obtained to analyze the retinoid concentration, expression of RXR-alpha, and beta-carotene 15,15'-dioxygenase activity. After UV exposure, the skin retinoid concentration was significantly lower as well as the expression of RXR-alpha. Higher skin beta-carotene dioxygenase activity was observed in the UV group as compared to the control group. We found no significant differences in the alpha-tocopherol concentration or acrolein levels in the skins of the two groups. In conclusion, the elevation of beta-carotene 15,15'-dioxygenase activity in hairless mice after UV exposure may be a response to reduction of the skin retinoid concentration.
- Subjects :
- Vitamin
Male
Antioxidant
medicine.drug_class
Ratón
Receptors, Retinoic Acid
Ultraviolet Rays
medicine.medical_treatment
Blotting, Western
alpha-Tocopherol
Medicine (miscellaneous)
chemistry.chemical_compound
Mice
Retinoids
beta-Carotene
medicine
Animals
Retinoid
Skin
beta-Carotene 15,15'-Monooxygenase
Mice, Hairless
Nutrition and Dietetics
integumentary system
Acrolein
Dioxygenase activity
Molecular biology
Hairless
Retinoid X Receptors
Biochemistry
chemistry
Liver
Oxygenases
Transcription Factors
Subjects
Details
- ISSN :
- 03014800
- Volume :
- 49
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of nutritional science and vitaminology
- Accession number :
- edsair.doi.dedup.....dba422182517a6619dcb5e059cad2fc6