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On the cDNA's for two types of rat pancreatic secretory trypsin inhibitor
- Source :
- Biochemical and biophysical research communications. 162(1)
- Publication Year :
- 1989
-
Abstract
- Two types of cDNA, which code for the two types of rat pancreatic secretory trypsin inhibitors (PSTIs), were cloned and sequenced. Both predicted amino acid sequences consisting of 79 amino acids, with the secretion signal peptide consisting of 18 and 23 amino acids for PSTI-I and PSTI-II, respectively. The nucleotide sequences were 91% homologous between the two cDNAs, but 68% and 65% homologous, respectively, when compared with human PSTI cDNA. Northern blot analyses showed that PSTI-I is expressed in the pancreas, whereas PSTI-II is expressed in the pancreas and the liver using the same promotor. Southern blot analyses suggested that both PSTI-I and PSTI-II genes are single copy genes per haploid genome. Duplication of rat PSTI gene seems to have occurred recently, after the divergene of humans and rats.
- Subjects :
- Signal peptide
Male
genetic processes
Molecular Sequence Data
Biophysics
Biochemistry
PstI
Complementary DNA
Sequence Homology, Nucleic Acid
medicine
Animals
Humans
Northern blot
Amino Acid Sequence
Cloning, Molecular
Growth Substances
Molecular Biology
Pancreatic Secretory Trypsin Inhibitor
Pancreas
Southern blot
chemistry.chemical_classification
biology
Base Sequence
Cell Biology
DNA
biochemical phenomena, metabolism, and nutrition
Trypsin
Molecular biology
Amino acid
Rats
chemistry
Genes
Trypsin Inhibitor, Kazal Pancreatic
biology.protein
bacteria
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Trypsin Inhibitors
medicine.drug
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 162
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....dba25696a188b1176e00e5e7a6ec4fe5