Back to Search
Start Over
Adipose TriGlyceride Lipase (ATGL) and Hormone-Sensitive Lipase (HSL) protein expression is decreased in the obese insulin resistant state
- Source :
- Journal of Clinical Endocrinology & Metabolism, 29(6), 2292-2299. Oxford University Press, Journal of Clinical Endocrinology and Metabolism, Journal of Clinical Endocrinology and Metabolism, 2007, 92 (6), pp.2292-9. ⟨10.1210/jc.2006-1318⟩, Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2007, 92 (6), pp.2292-9. ⟨10.1210/jc.2006-1318⟩
- Publication Year :
- 2007
-
Abstract
- International audience; AIM/HYPOTHESIS: Obesity is associated with increased triacylglycerol (TAG) storage in adipose tissue and insulin resistance. The mobilization of stored TAG is mediated by hormone-sensitive lipase (HSL) and the recently discovered adipose triglyceride lipase (ATGL). The aim of the present study was to examine whether ATGL and HSL mRNA and protein expression are altered in insulin-resistant conditions. In addition, we investigated whether a possible impaired expression could be reversed by a period of weight reduction. METHODS: Adipose tissue biopsies were taken from obese subjects (n = 44) with a wide range of insulin resistance, before and just after a 10-wk hypocaloric diet. ATGL and HSL protein and mRNA expression was determined by Western blot and quantitative RT-PCR, respectively. RESULTS: Fasting insulin levels and the degree of insulin resistance (using the homeostasis model assessment index for insulin resistance) were negatively correlated with ATGL and HSL protein expression, independent of age, gender, fat cell size, and body composition. Both mRNA and protein levels of ATGL and HSL were reduced in insulin-resistant compared with insulin-sensitive subjects (P < 0.05). Weight reduction significantly decreased ATGL and HSL mRNA and protein expression. A positive correlation between the decrease in leptin and the decrease in ATGL protein level after weight reduction was observed. Finally, ATGL and HSL mRNA and protein levels seem to be highly correlated, indicating a tight coregulation and transcriptional control. CONCLUSIONS: In obese subjects, insulin resistance and hyperinsulinemia are strongly associated with ATGL and HSL mRNA and protein expression, independent of fat mass. Data on weight reduction indicated that also other factors (e.g. leptin) relate to ATGL and HSL protein expression.
- Subjects :
- Male
Biopsy
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Adipose tissue
Hormone-sensitive lipase
MESH: Regression Analysis
Biochemistry
MESH: Lipase
MESH: Biopsy
0302 clinical medicine
Endocrinology
Hyperinsulinemia
MESH: Obesity
MESH: Diet, Reducing
2. Zero hunger
0303 health sciences
MESH: Middle Aged
MESH: Sterol Esterase
MESH: Gene Expression Regulation, Enzymologic
Leptin
food and beverages
Middle Aged
MESH: Insulin Resistance
Adipose Tissue
MESH: Hyperinsulinism
Regression Analysis
Female
MESH: Adipose Tissue
Adult
medicine.medical_specialty
Diet, Reducing
030209 endocrinology & metabolism
Biology
Gene Expression Regulation, Enzymologic
MESH: Weight Loss
03 medical and health sciences
Insulin resistance
Hyperinsulinism
Internal medicine
Weight Loss
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
medicine
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Obesity
RNA, Messenger
MESH: RNA, Messenger
030304 developmental biology
MESH: Humans
Insulin
Biochemistry (medical)
MESH: Adult
Lipase
Sterol Esterase
medicine.disease
MESH: Male
Adipose triglyceride lipase
Insulin Resistance
MESH: Female
Subjects
Details
- Language :
- English
- ISSN :
- 0021972X and 19457197
- Volume :
- 29
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....dba2503dbbcb591f78645ccdc8582b7d