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Effect of training frequency on endothelium-dependent vasorelaxation in rats
- Source :
- European Journal of Cardiovascular Prevention and Rehabilitation, European Journal of Cardiovascular Prevention and Rehabilitation, SAGE Publications, 2008, 15 (1), pp.52-8. ⟨10.1097/HJR.0b013e3282f17baa⟩
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- International audience; BACKGROUND: Moderate physical activity enhances endothelium-dependent vasorelaxation. Whether the frequency of exercise affects endothelial function is unclear. The purpose of this study was to investigate the effects of various frequencies of training on endothelium-dependent vasorelaxation. DESIGN: Male Wistar rats were trained for 8 weeks on a treadmill at various frequencies [1 (Ex1), 3 (Ex3) or 5 days/week (Ex5)] and compared with age-matched sedentary animals (SED). A control group allowed us to assess endothelial function before the exercise protocol. Rings of thoracic aorta were precontracted with phenylephrine. RESULTS: Endothelium-independent relaxation elicited by sodium nitroprusside was similar in all groups. The maximal response elicited by acetylcholine (ACh) was not different between groups, whereas pD2 values (-logEC50, EC50 being the concentration of ACh that elicited 50% of the maximal response) significantly correlated with frequency of training. nitro-L-arginine methyl ester (L-NAME) reduced the relaxation elicited by 10(-7) mol/l ACh or higher in control and all trained groups, and by 10(-6) mol/l ACh or higher in SED group. Indomethacin inhibited the vasodilating response to 10(-7) mol/l ACh or higher in control, SED and Ex1 groups, and to 10(-8) mol/l or more in Ex3 and Ex5 animals. Tetraethylammonium attenuated the response to 10(-6) mol/l ACh or higher in control and SED groups and to 10(-7) mol/l or more in all trained animals. CONCLUSION: This data suggest that decreased ACh-induced vasorelaxation after physical inactivity may result from impairment of endothelial nitric oxide synthase, prostacyclin and endothelium-derived hyperpolarizing factor pathways. This effect is prevented by training in a frequency-dependent manner.
- Subjects :
- Male
MESH: Phenylephrine
Arginine
Epidemiology
MESH: Random Allocation
Indomethacin
Vasodilation
MESH: Physical Conditioning, Animal
030204 cardiovascular system & hematology
MESH: Dose-Response Relationship, Drug
Phenylephrine
Random Allocation
MESH: NG-Nitroarginine Methyl Ester
0302 clinical medicine
rat
MESH: Animals
MESH: Indomethacin
0303 health sciences
Tetraethylammonium
3. Good health
NG-Nitroarginine Methyl Ester
medicine.anatomical_structure
Anesthesia
MESH: Citrate (si)-Synthase
MESH: Endothelium, Vascular
Sodium nitroprusside
Cardiology and Cardiovascular Medicine
Acetylcholine
medicine.drug
Nitroprusside
medicine.medical_specialty
endothelium
MESH: Rats
Endothelium
Citrate (si)-Synthase
Endothelium dependent
NO
MESH: Vasodilation
03 medical and health sciences
EDHF
Physical Conditioning, Animal
MESH: Analysis of Variance
Internal medicine
medicine.artery
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
medicine
Animals
Rats, Wistar
030304 developmental biology
Analysis of Variance
Aorta
Dose-Response Relationship, Drug
MESH: Acetylcholine
business.industry
COX
MESH: Rats, Wistar
biochemical phenomena, metabolism, and nutrition
acetylcholine
MESH: Male
MESH: Nitroprusside
Rats
aorta
Endocrinology
MESH: Tetraethylammonium
Endothelium, Vascular
business
exercise training
Subjects
Details
- ISSN :
- 17418267
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- European Journal of Cardiovascular Prevention & Rehabilitation
- Accession number :
- edsair.doi.dedup.....db8ffb3d18977993f84938fb8fba5b07