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Role of ADP-ribosylation factor 6 (ARF6) in gastric acid secretion
- Source :
- The Journal of biological chemistry. 278(38)
- Publication Year :
- 2003
-
Abstract
- ADP-ribosylation factor (ARF) proteins are monomeric GTPases that are essential for membrane transport and exocytosis in a number of secretory cells. We investigated ARF6, the activation of which is insensitive to brefeldin A, to determine whether it regulates membrane traffic in gastric parietal cells. ARF6 translocated from cytosol to tubulovesicle in the presence of GTPgammaS, a potential inhibitor of acid secretion in permeabilized cells, whereas under the Mg2+-chelated condition where activity of ARF-GTPase activating protein is inhibited, ARF6 translocated to the apical secretory membrane. Immunohistochemical examination revealed that ARF6 mainly located in parietal cell within the gastric glands, and it translocated from the cytosol to the intracellular canaliculi when the glands were stimulated. These results indicated that the distribution of ARF6 between cytosol and the two different membranes was regulated by its GTPase activity. In cultured gastric glands infected with adenovirus expressing ARF6 Q67L, a mutant lacking GTP hydrolysis activity, gastric acid secretion was inhibited. These results suggest that ARF6 regulates gastric acid secretion in parietal cell and that the GTP hydrolysis cycle of ARF6 is essential for the activation pathway.
- Subjects :
- GTPgammaS
Biology
Biochemistry
Exocytosis
Adenoviridae
Gastric Acid
chemistry.chemical_compound
H(+)-K(+)-Exchanging ATPase
Cytosol
Gastric glands
medicine
Animals
Secretion
Magnesium
Enterochromaffin-like cell
Molecular Biology
Parietal cell
Protein Synthesis Inhibitors
Brefeldin A
ADP-Ribosylation Factors
Hydrolysis
Cell Membrane
Cell Biology
Molecular biology
Immunohistochemistry
Cell biology
Gastric chief cell
Protein Transport
medicine.anatomical_structure
chemistry
ADP-Ribosylation Factor 6
Gastric Mucosa
Guanosine 5'-O-(3-Thiotriphosphate)
Mutation
Gastric acid
Rabbits
Peptides
Histamine
Subcellular Fractions
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 278
- Issue :
- 38
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....db86fdd9479e9ddfe235fafa2d697bf8