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All-sealed paper-based electrochemiluminescence platform for on-site determination of lead ions
- Source :
- Biosensors and Bioelectronics. 192:113524
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Lab-on-paper (LOP) devices are urgently required for the rapid development of point-of-care diagnoses and environmental assays. Herein, an all-sealed paper-based electrochemiluminescence (ECL) platform was developed to achieve lead ions (Pb2+) sensitive analysis via incorporating convenient plastic package technology. Benefiting from transparent plastic encapsulation, the sealed devices effectively avoided the interference of O2. Meanwhile, myrica rubra-like Pt nanomaterials (MPNs) prepared by an economical and easy-to-operate ultrasound method were employed to catalyze H2O2 decomposition. With the help of Pb2+-specific DNAzymes, the oligonucleotide probe functionalized via MPNs could be detached from the device in the presence of target, resulting in the reduced ECL intensity. Moreover, the combination of modified paper electrode with functional regions separated by multiple layers of wax enhanced the practicability of the LOP device for rapid detection. Under the optimal conditions, the all-sealed platform achieved wide linear relationship ranging from 0.01 nM to 0.05 μM with a low detection limit of 0.004 nM for sensitive detecting Pb2+. It is believed that this platform could provide a robust, simple and versatile strategy for sensitive determination of heavy metal ions, and be applied in on-site contamination analysis in the future.
- Subjects :
- Ions
Detection limit
Materials science
Metal ions in aqueous solution
Biomedical Engineering
Biophysics
Deoxyribozyme
Nanotechnology
Biosensing Techniques
DNA, Catalytic
Electrochemical Techniques
Hydrogen Peroxide
General Medicine
Ion
Nanomaterials
Lead
Interference (communication)
Limit of Detection
Luminescent Measurements
Electrode
Electrochemistry
Electrochemiluminescence
Biotechnology
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....db7f706a561d3a87002ef5f800fee0e6