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A pan-plant protein complex map reveals deep conservation and novel assemblies
- Source :
- Cell, vol 181, iss 2
- Publication Year :
- 2019
- Publisher :
- Cold Spring Harbor Laboratory, 2019.
-
Abstract
- SUMMARYPlants are foundational to global ecological and economic systems, yet most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. Using co-fractionation mass spectrometry, we recovered known complexes, confirmed complexes predicted to occur in plants, and identified novel interactions conserved over 1.1 billion years of green plant evolution. Several novel complexes are involved in vernalization and pathogen defense, traits critical to agriculture. We also uncovered plant analogs of animal complexes with distinct molecular assemblies, including a megadalton-scale tRNA multi-synthetase complex. The resulting map offers the first cross-species view of conserved, stable protein assemblies shared across plant cells and provides a mechanistic, biochemical framework for interpreting plant genetics and mutant phenotypes.
- Subjects :
- Proteomics
0106 biological sciences
Plant genetics
Mutant
co-fractionation mass spectrometry
Computational biology
Biology
protein interactions
Medical and Health Sciences
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Mass Spectrometry
Protein–protein interaction
comparative proteomics
03 medical and health sciences
0302 clinical medicine
evolution
Protein Interaction Mapping
Genetics
Protein Interaction Maps
Gene
Plant Proteins
030304 developmental biology
2. Zero hunger
Plant evolution
0303 health sciences
protein complexes
pathogen defense
plants
cross-linking mass spectrometry
fungi
food and beverages
Vernalization
interaction-to-phenotype
Biological Sciences
Plants
15. Life on land
Phenotype
Plant protein
Transfer RNA
Generic health relevance
030217 neurology & neurosurgery
Developmental Biology
010606 plant biology & botany
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Cell, vol 181, iss 2
- Accession number :
- edsair.doi.dedup.....db7891ee977a1271ca5aaa53258285eb