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The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair
- Source :
- eLife, Vol 10 (2021), eLife
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications, Ltd, 2021.
-
Abstract
- Meniscal tears are associated with a high risk of osteoarthritis but currently have no disease-modifying therapies. Using a Gli1 reporter line, we found that Gli1+ cells contribute to the development of meniscus horns from 2 weeks of age. In adult mice, Gli1+ cells resided at the superficial layer of meniscus and expressed known mesenchymal progenitor markers. In culture, meniscal Gli1+ cells possessed high progenitor activities under the control of Hh signal. Meniscus injury at the anterior horn induced a quick expansion of Gli1-lineage cells. Normally, meniscal tissue healed slowly, leading to cartilage degeneration. Ablation of Gli1+ cells further hindered this repair process. Strikingly, intra-articular injection of Gli1+ meniscal cells or an Hh agonist right after injury accelerated the bridging of the interrupted ends and attenuated signs of osteoarthritis. Taken together, our work identified a novel progenitor population in meniscus and proposes a new treatment for repairing injured meniscus and preventing osteoarthritis.
- Subjects :
- Male
0301 basic medicine
Pathology
Time Factors
Mouse
Swine
Gli1
meniscus injury
Osteoarthritis
Meniscus (anatomy)
Menisci, Tibial
Regenerative medicine
0302 clinical medicine
Medicine
Biology (General)
Mice, Knockout
education.field_of_study
integumentary system
General Neuroscience
General Medicine
Osteoarthritis, Knee
musculoskeletal system
Stem Cells and Regenerative Medicine
Tibial Meniscus Injuries
medicine.anatomical_structure
030220 oncology & carcinogenesis
Swine, Miniature
Stem cell
Signal Transduction
Research Article
medicine.medical_specialty
QH301-705.5
Science
Population
Mesenchymal Stem Cell Transplantation
Zinc Finger Protein GLI1
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
lineage tracing
Animals
Humans
Cell Lineage
Hedgehog Proteins
Progenitor cell
education
Cell Proliferation
Progenitor
Wound Healing
General Immunology and Microbiology
business.industry
mesenchymal progenitor
Mesenchymal stem cell
Mesenchymal Stem Cells
medicine.disease
Disease Models, Animal
osteoarthritis
030104 developmental biology
business
Developmental Biology
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....db77fad850b2b947a726aadfde989a02
- Full Text :
- https://doi.org/10.7554/elife.62917