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Characterization of serum metabolites as biomarkers of carbon black nanoparticles-induced subchronic toxicity in rats by hybrid triple quadrupole time-of-flight mass spectrometry with non-targeted metabolomics strategy
- Source :
- Toxicology. 426
- Publication Year :
- 2019
-
Abstract
- Carbon black nanoparticles (CBNPs) are one of atmospheric particles components and have been closely related with a series of lung diseases. It can reach the depths of the respiratory tract or even alveolar more easily than those micro-particles. Although some of its toxicities have been confirmed in animals or human bodies, the subchronic toxicity mechanism of CBNPs has been uncertain so far. Therefore, it is very necessary to establish a novel method and clarify the mechanism of subchronic toxicity caused by concentration adjustments of small molecule metabolites in vivo. In animal experiments, CB exposure, recovery and control group were set up. The concentration of CBNPs in chamber was 30.06 ± 4.42 mg/m3. We developed a UHPLC-Q-TOF-MS/MS-based non-targeted metabolomic analysis strategy to analyze serum samples of rats. Then, differential metabolites in serum were found by multivariate data analysis and 39 potential biomarkers were identified. It was showed that main metabolic pathways associated with CBNPs exposure were hormones metabolism, amino acid metabolism, nucleotide metabolism and lipid metabolism. It is worth noting that long-term exposure to CBNPs had the greatest impact on steroid hormones biosynthesis so that the risk of infertility could increase. The results provided a new mechanistic insight into the metabolic alterations owing to CBNPs induced subchronic toxicity.
- Subjects :
- 0301 basic medicine
Lung Diseases
Male
Atmosphere Exposure Chambers
medicine.medical_treatment
Pharmacology
Toxicology
Steroid
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Metabolomics
Biosynthesis
Soot
In vivo
Tandem Mass Spectrometry
medicine
Animals
Hormone metabolism
Amino Acids
Chemistry
Nucleotides
Lipid metabolism
Lipid Metabolism
Hormones
Rats
Metabolic pathway
030104 developmental biology
Particulate Matter
030217 neurology & neurosurgery
Biomarkers
Metabolic Networks and Pathways
Hormone
Subjects
Details
- ISSN :
- 18793185
- Volume :
- 426
- Database :
- OpenAIRE
- Journal :
- Toxicology
- Accession number :
- edsair.doi.dedup.....db5d400e6ce118e47ecf696b3ad24bb8