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Haemophilus influenzae porine ompP2 gene transfer mediated by graphene oxide nanoparticles with effects on transformation process and virulence bacterial capacity
- Source :
- Journal of Nanobiotechnology
- Publication Year :
- 2014
- Publisher :
- BioMed Central, 2014.
-
Abstract
- Background H. influenzae is a natural competent bacterium that can uptake DNA from the environment and recombine into bacterial genome. The outbreaks of Brazilian purpuric fever, heavily polluted areas of a different H. influenzae biogroup - aegyptius - as well as gene transference between Neisseria meningitis make the transformation process an important evolutionary factor. This work studied the horizontal transference of the ompP2 gene from a multiresistant strain of H. influenzae 07 (NTHi), under the influence of graphene oxide nanoparticles in order to mimic an atmosphere rich in suspended particles and this way verify if the CFU transformants number was increased. Material and methods In this article the gene ompP2 was transformed into different strains of H. influenzae mediated or not by graphene oxide nanoparticles in suspension, followed by the adhesion tests in Hec-1B (human endometrium adenocarcinoma) and A549 (pulmonary epithelial carcinoma) cells lines. The transformation frequency and the adhesion capacity were determined in all the mutants to which the ompP2 gene was transferred and compared to their wild type strains. Results The nanoparticles increased the transformation ratio of one particular strain isolated from a pneumonia case. The adhesion patterns to A549 and Hec1b cell lines of these mutated bacteria has their capacity increased when compared to the wild type. Conclusions Graphene oxide nanoparticles aid the transformation process, helping to increase the number of CFUs, and the mutants generated with the ompP2 gene from a H. influenzae resistant strain not only present a chloramphenicol resistance but also have an increased adherence patterns in A549 and Hec1B cell lines.
- Subjects :
- Haemophilus Infections
Mutant
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Virulence
Porins
Bioengineering
medicine.disease_cause
Applied Microbiology and Biotechnology
Bacterial Adhesion
Haemophilus influenzae
Microbiology
Bacterial Proteins
Cell Line, Tumor
medicine
Humans
Brazilian purpuric fever
Graphene oxide
Bacterial transformation
biology
Research
Porin
Wild type
Oxides
medicine.disease
biology.organism_classification
Transformation (genetics)
Outer membrane protein
Host-Pathogen Interactions
Mutation
Molecular Medicine
Nanoparticles
Graphite
Neisseria
Transformation, Bacterial
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 14773155
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Nanobiotechnology
- Accession number :
- edsair.doi.dedup.....db5aeeaa7296fd6f96efe3a0e6ae9f6b