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BP180 ELISA using bacterial recombinant NC16a protein as a diagnostic and monitoring tool for bullous pemphigoid
- Source :
- Journal of Dermatological Science. 30:224-232
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- Bullous pemphigoid (BP) is an acquired autoimmune subepidermal blistering disease against hemidesmosomal cytoplasmic BP230 and transmembrane BP180 proteins. Epitope mapping studies have shown that the membrane-proximal noncollagenous (NC) 16a domain of BP180 harbors clusters of antigenic sites recognized by the vast majority of BP sera. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) using bacterial recombinant NC16a protein and evaluated its clinical benefit for diagnosis and monitoring disease activity. Fifty four (84.4%) of 64 sera from BP patients were positive, while only one (1.1%) of 91 sera from collagen disease patients and five (1.5%) of 336 sera from normal control barely exceeded the cut-off value. None of 69 pemphigus vulgaris sera and none of 42 pemphigus foliaceus sera exceeded the cut-off value. Thus, the sensitivity and specificity of NC16a ELISA were 84.4 and 98.9%, respectively. The correlation between ELISA scores and disease activity along the time course was examined using seven BP patients. NC16a ELISA scores tended to fluctuate in parallel with the disease activity along the time course and reflected the disease activity much better than indirect immunofluorescence. These findings indicate that NC16a ELISA will be a valuable tool not only for the diagnosis of patients with BP but also for the monitoring of the disease activity.
- Subjects :
- Adult
Male
Pemphigoid
Dystonin
Enzyme-Linked Immunosorbent Assay
Nerve Tissue Proteins
Dermatology
Autoantigens
Sensitivity and Specificity
Biochemistry
Bacterial Proteins
Antigen
Pemphigoid, Bullous
Humans
Medicine
Molecular Biology
Pemphigus foliaceus
Aged
Aged, 80 and over
Collagen disease
business.industry
Pemphigus vulgaris
Collagen Diseases
Autoantibody
Middle Aged
Non-Fibrillar Collagens
medicine.disease
Recombinant Proteins
Protein Structure, Tertiary
Cytoskeletal Proteins
Epitope mapping
Case-Control Studies
Immunology
Female
Collagen
Bullous pemphigoid
Carrier Proteins
business
Subjects
Details
- ISSN :
- 09231811
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Journal of Dermatological Science
- Accession number :
- edsair.doi.dedup.....db512125bff1aacc3867e42f2128440d