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Involvement of glycosaminoglycans in detachment of early myeloid precursors from bone-marrow stromal cells
- Source :
- Biochimica et biophysica acta. 676(2)
- Publication Year :
- 1981
-
Abstract
- Fibroblast-like cells were obtained by in vitro cultivation of needle aspirations of human bone-marrow. These cells show a unique composition of coat-associated glycosaminoglycans: 10% chondroitin 4-sulfate, 30% hyaluronic acid and 60% heparan sulfate which were resolved and characterized by electrophoresis, nitrous acid treatment and enzymatic degradation. Chondroitin 4-sulfate is the only glycoaminoglycan detectable on the surface of mature granulocytes, whereas the immature cells do not seem to possess surface glycoaminoglycans. Immature hemopoietic cells can adhere on to marrow-derived fibroblast cells, whereas mature granulocytes cannot. Treatment with mucopolysaccharidases of both mature leukocytes and marrow stromal cells can interfere in these adhesive relationships, suggesting a role of glycoaminoglycans in regulating short-range interactions during hematopoiesis.
- Subjects :
- Myeloid
Stromal cell
Biophysics
Hyaluronoglucosaminidase
Bone Marrow Cells
Biology
Biochemistry
chemistry.chemical_compound
Hyaluronic acid
medicine
Cell Adhesion
Leukocytes
Chondroitin
Humans
Hyaluronic Acid
Molecular Biology
Glycosaminoglycans
Polysaccharide-Lyases
Leukemia
Chondroitin Sulfates
Heparan sulfate
Fibroblasts
Cell biology
Haematopoiesis
medicine.anatomical_structure
chemistry
Bone marrow
Myelopoiesis
Heparitin Sulfate
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 676
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....db3656959e099569ef27e974fbfe0be8