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Involvement of glycosaminoglycans in detachment of early myeloid precursors from bone-marrow stromal cells

Authors :
Gabriella Fibbi
S Vannucchi
Vincenzo Chiarugi
M Delrosso
C Guazzelli
Renzo Cappelletti
G Dini
Source :
Biochimica et biophysica acta. 676(2)
Publication Year :
1981

Abstract

Fibroblast-like cells were obtained by in vitro cultivation of needle aspirations of human bone-marrow. These cells show a unique composition of coat-associated glycosaminoglycans: 10% chondroitin 4-sulfate, 30% hyaluronic acid and 60% heparan sulfate which were resolved and characterized by electrophoresis, nitrous acid treatment and enzymatic degradation. Chondroitin 4-sulfate is the only glycoaminoglycan detectable on the surface of mature granulocytes, whereas the immature cells do not seem to possess surface glycoaminoglycans. Immature hemopoietic cells can adhere on to marrow-derived fibroblast cells, whereas mature granulocytes cannot. Treatment with mucopolysaccharidases of both mature leukocytes and marrow stromal cells can interfere in these adhesive relationships, suggesting a role of glycoaminoglycans in regulating short-range interactions during hematopoiesis.

Details

ISSN :
00063002
Volume :
676
Issue :
2
Database :
OpenAIRE
Journal :
Biochimica et biophysica acta
Accession number :
edsair.doi.dedup.....db3656959e099569ef27e974fbfe0be8