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In vivo digoxin-like immunoreactivity in mice and interference of Chinese medicine Danshen in serum digoxin measurement: elimination of interference by using a chemiluminescent assay
- Source :
- Clinica Chimica Acta. 317:231-234
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- Background: Danshen, a traditional Chinese medicine used in the management of cardiovascular diseases, is available without prescription in the US. Because Danshen is used to treat cardiovascular diseases, we studied the potential interference of Danshen with serum digoxin measurement using various immunoassays. Methods: Blood was collected 1 day before and then 1 and 2 h after feeding mice with Danshen. The apparent digitoxin activities were measured by the fluorescence polarization immunoassay (FPIA). We also added microliter amounts of Danshen extract to digoxin pools prepared from patients receiving digoxin. The digoxin concentrations were measured using the fluorescence polarization immunoassay (FPIA), microparticle enzyme immunoassay (MEIA) and chemiluminescent assay (CLIA). The observed values were compared with original values. We also fed mice with Danshen. Results: We observed measurable digoxin-like immunoreactivity in sera of mice after feeding with Danshen. We also observed falsely lower digoxin concentrations (negative interference) when MEIA was used for digoxin measurement. However, serum digoxin concentrations were falsely elevated with FPIA. We observed no interference of Danshen in serum digoxin measurement using the CLIA. Conclusions: Danshen appears to contain digoxin-like immunoreactivity but does not interfere with serum digoxin measurement when CLIA was used.
- Subjects :
- Digoxin
Digitoxin
Clinical Biochemistry
Salvia miltiorrhiza
Microparticle Enzyme Immunoassay
Traditional Chinese medicine
Pharmacology
Biochemistry
law.invention
Immunoenzyme Techniques
Mice
law
In vivo
Fluorescence Polarization Immunoassay
polycyclic compounds
medicine
Animals
Humans
cardiovascular diseases
Medicine, Chinese Traditional
Chemiluminescence
Immunoassay
Plant Extracts
Chemistry
digestive, oral, and skin physiology
Biochemistry (medical)
General Medicine
carbohydrates (lipids)
Luminescent Measurements
Artifacts
Quantitative analysis (chemistry)
circulatory and respiratory physiology
medicine.drug
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 317
- Database :
- OpenAIRE
- Journal :
- Clinica Chimica Acta
- Accession number :
- edsair.doi.dedup.....db2d54eb860cac114b2588dec283f2a1