Back to Search
Start Over
A parallel evaluation of 5 indirect cost-effective methods for assessing failure of passive immunity transfer in neonatal calves
- Source :
- JDS communications. 1(1)
- Publication Year :
- 2019
-
Abstract
- The aim of this study was to evaluate the diagnostic accuracy of 5 indirect methods that may be used in veterinary practices to assess the success of maternal immunoglobulins transfer in neonatal calves. Blood samples (n = 245) were collected from 2- to 6-d-old calves. Serum immunoglobulin concentrations were determined by radial immunodiffusion. Serum total protein and albumin concentrations and γ-glutamyl transferase activity were determined by veterinary practitioners using their own biochemistry analyzer. Globulin concentration was calculated. Veterinary practitioners were also asked to assess serum total protein using a digital Brix refractometer (%Brix) and an optical refractometer (g/L). Test sensitivity (Se) and specificity (Sp) were calculated using radial immunodiffusion as the reference standard, with failure of transfer of passive immunity defined as IgG10.0 g/L. Receiver operating characteristics curves were created and optimal cutoff values for each of the 5 indirect measurement methods were selected based on the maximization of the Youden's
Details
- ISSN :
- 26669102
- Volume :
- 1
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- JDS communications
- Accession number :
- edsair.doi.dedup.....db22eb4fab842509a47f6b87e358737a