Back to Search
Start Over
The effects of reduced rpd3 levels on fly physiology
- Source :
- Nutrition and Healthy Aging
- Publication Year :
- 2017
- Publisher :
- IOS Press, 2017.
-
Abstract
- BACKGROUND: Rpd3 is a conserved histone deacetylase that removes acetyl groups from lysine residues within histones and other proteins. Reduction or inhibition of Rpd3 extends longevity in yeast, worms, and flies. Previous studies in flies suggest an overlap with the mechanism of lifespan extension by dietary restriction. However, the mechanism of rpd3's effects on longevity remains unclear. OBJECTIVES: In this study we investigated how rpd3 reduction affects fly spontaneous physical activity, fecundity, and stress resistance. METHODS: We examined the effects of rpd3 reduction on fly spontaneous physical activity by using population monitors, we determined female fecundity by counting daily egg laying, and we determined fly survivorship in response to starvation and paraquat. RESULTS: In flies, rpd3 reduction increases peak spontaneous physical activity of rpd3 def male flies at a young age but does not affect total 24 hour activity. Male and female rpd3 def mutants are more resistant to starvation on low and high calorie diets. In addition, increased resistance to paraquat was observed in females of one allele. A decrease in rpd3 levels does not affect female fecundity. CONCLUSIONS: A decrease in rpd3 levels mirrors some but not all changes associated with calorie restriction, illustrated by an increased peak of spontaneous activity in rpd3 def /+ heterozygous male flies but no effect on total spontaneous activity and fecundity.
- Subjects :
- Research Report
medicine.medical_specialty
media_common.quotation_subject
Calorie restriction
Population
Medicine (miscellaneous)
Biology
Biochemistry
rpd3
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Paraquat
Internal medicine
medicine
Allele
education
030304 developmental biology
media_common
2. Zero hunger
0303 health sciences
education.field_of_study
Nutrition and Dietetics
aging
fungi
Longevity
dietary restriction
Fecundity
biology.organism_classification
Drosophila melanogaster
Endocrinology
chemistry
Histone deacetylase
030217 neurology & neurosurgery
Food Science
Subjects
Details
- ISSN :
- 24519502 and 24519480
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Nutrition and Healthy Aging
- Accession number :
- edsair.doi.dedup.....db218863bcb55c9c1e303756192cf55f