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Single-reaction multi-antigen serological test for comprehensive evaluation of SARS-CoV-2 patients by flow cytometry
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, European Journal of Immunology
- Publication Year :
- 2021
- Publisher :
- John Wiley & Sons, 2021.
-
Abstract
- Here, we describe a new, simple, highly multiplexed serological test that generates a more complete picture of seroconversion than single antigen-based assays. Flow cytometry is used to detect multiple Ig isotypes binding to four SARS-CoV-2 antigens: the Spike glycoprotein, its RBD fragment (the main target for neutralizing antibodies), the nucleocapsid protein, and the main cysteine-like protease in a single reaction. Until now, most diagnostic serological tests measured antibodies to only one antigen and in some laboratory-confirmed patients no SARS-CoV-2-specific antibodies could be detected. Our data reveal that while most patients respond against all the viral antigens tested, others show a marked bias to make antibodies against either proteins exposed on the viral particle or those released after cellular infection. With this assay, it was possible to discriminate between patients and healthy controls with 100% confidence. Analysing the response of multiple Ig isotypes to the four antigens in combination may also help to establish a correlation with the severity degree of disease. A more detailed description of the immune responses of different patients to SARS-CoV-2 virus might provide insight into the wide array of clinical presentations of COVID-19.<br />This work was supported by: Spanish National Research Council (CSIC-202020E079, CSIC-COVID19-028); Madrid Regional Government “IMMUNOTHERCAN” [S2017/BMD-3733-2 (MVG)]; Spanish Ministry of Science and Innovation [(MCIU/AEI/FEDER, EU, RTI2018-093569-B-I00 (MVG), SAF2017-82940-R (JMRF), SAF2017-83265-R (HTR); SAF2017-82886-R (FSM)]; Health Institute Carlos III (ISCIII) [RETICS Program RD16/0012/0006; RIER (EMGC); PI19/00549 (AA)]; “La Caixa Bank Foundation” (HR17-00016), Fondo Supera COVID (CRUE-Banco de Santander), both to FSM.
- Subjects :
- Short Communication|Clinical
Short Communication
Immunology
FACS
Immunity to infection
coronavirus
serology
Biology
Antibodies, Viral
medicine.disease_cause
Sensitivity and Specificity
Virus
COVID-19 Serological Testing
Flow cytometry
Serology
Microbeads
Clinical
Immune system
Antigen
COVID‐19
diagnostics
medicine
Humans
Immunology and Allergy
Serologic Tests
Seroconversion
Antigens, Viral
Diagnostics
Coronavirus
medicine.diagnostic_test
SARS-CoV-2
COVID-19
Flow Cytometry
High-Throughput Screening Assays
biology.protein
microbeads
Antibody
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....db1e0f2d0c38a8235d0a3c380f5da0a9