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Respiratory Syncytial Virus Human Experimental Infection Model: Provenance, Production, and Sequence of Low-Passaged Memphis-37 Challenge Virus
- Source :
- PLoS ONE, Vol 9, Iss 11, p e113100 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in children and is responsible for as many as 199,000 childhood deaths annually worldwide. To support the development of viral therapeutics and vaccines for RSV, a human adult experimental infection model has been established. In this report, we describe the provenance and sequence of RSV Memphis-37, the low-passage clinical isolate used for the model's reproducible, safe, experimental infections of healthy, adult volunteers. The predicted amino acid sequences for major proteins of Memphis-37 are compared to nine other RSV A and B amino acid sequences to examine sites of vaccine, therapeutic, and pathophysiologic interest. Human T- cell epitope sequences previously defined by in vitro studies were observed to be closely matched between Memphis-37 and the laboratory strain RSV A2. Memphis-37 sequences provide baseline data with which to assess: (i) virus heterogeneity that may be evident following virus infection/transmission, (ii) the efficacy of candidate RSV vaccines and therapeutics in the experimental infection model, and (iii) the potential emergence of escape mutants as a consequence of experimental drug treatments. Memphis-37 is a valuable tool for pre-clinical research, and to expedite the clinical development of vaccines, therapeutic immunomodulatory agents, and other antiviral drug strategies for the protection of vulnerable populations against RSV disease.
- Subjects :
- CD4-Positive T-Lymphocytes
Male
Epitopes, T-Lymphocyte
lcsh:Medicine
CD8-Positive T-Lymphocytes
Epitope
Chlorocebus aethiops
lcsh:Science
Vaccines
Multidisciplinary
Respiratory tract infections
Transmission (medicine)
Viral Immune Evasion
Viral Vaccine
Hep G2 Cells
Viral Load
Vaccination and Immunization
Tennessee
3. Good health
Treatment Outcome
Child, Preschool
Host-Pathogen Interactions
Bronchiolitis
Viral Clearance
Research Article
medicine.drug_class
Immunology
Molecular Sequence Data
Antiviral Therapeutics
Respiratory Syncytial Virus Infections
Biology
Microbiology
Antiviral Agents
Virus
Viral Proteins
Antiviral Therapy
Virology
Vaccine Development
Respiratory Syncytial Virus Vaccines
medicine
Animals
Humans
Amino Acid Sequence
Vero Cells
Immune Evasion
Sequence Homology, Amino Acid
lcsh:R
Biology and Life Sciences
Viral Vaccines
medicine.disease
Animal Models of Infection
Respiratory Syncytial Virus, Human
Viral Genes
Clinical Immunology
lcsh:Q
Antiviral drug
Viral Transmission and Infection
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....db0e8586799b7d7d3866c56ccf79d852