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Quantitative analysis of trace amounts of estrogenic steroids in pregnancy urine by column liquid-liquid chromatography with ultraviolet detection

Authors :
J.F.K. Huber
J. A. R. J. Hulsman
C. A. M. Meijers
Source :
Journal of chromatography. 62(1)
Publication Year :
1971

Abstract

The influence of the sample volume on peak height and resolution in elution column chromatography was studied. Graphs which enable the choice of the optimum sample volume are given. The quantitative analysis of estrogens by column liquid–liquid chromatography with ultraviolet detection was investigated. The method has been applied to the rapid quantitative determination of estriol in urine. After preparing the urine sample for chromatography, the quantitative information can be obtained in 20 min with a precision of 5″6 for a concentration of 3 mg l. The noise of the sample-free detector corresponds to 60 ng of estriol.

Details

Volume :
62
Issue :
1
Database :
OpenAIRE
Journal :
Journal of chromatography
Accession number :
edsair.doi.dedup.....db0d8e6c4b7f4e97aa665f0e9aefa9bd