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Quantitative analysis of trace amounts of estrogenic steroids in pregnancy urine by column liquid-liquid chromatography with ultraviolet detection
- Source :
- Journal of chromatography. 62(1)
- Publication Year :
- 1971
-
Abstract
- The influence of the sample volume on peak height and resolution in elution column chromatography was studied. Graphs which enable the choice of the optimum sample volume are given. The quantitative analysis of estrogens by column liquid–liquid chromatography with ultraviolet detection was investigated. The method has been applied to the rapid quantitative determination of estriol in urine. After preparing the urine sample for chromatography, the quantitative information can be obtained in 20 min with a precision of 5″6 for a concentration of 3 mg l. The noise of the sample-free detector corresponds to 60 ng of estriol.
- Subjects :
- Resolution (mass spectrometry)
Trace Amounts
Estrone
Ultraviolet Rays
Urine
medicine.disease_cause
Biochemistry
Analytical Chemistry
Column chromatography
Pregnancy
medicine
Methods
Humans
Chromatography
Autoanalysis
Estradiol
Elution
Chemistry
Estriol
Organic Chemistry
Estrogens
General Medicine
Chromatography, Ion Exchange
Spectrophotometry
Female
Quantitative analysis (chemistry)
Ultraviolet
Mathematics
Subjects
Details
- Volume :
- 62
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography
- Accession number :
- edsair.doi.dedup.....db0d8e6c4b7f4e97aa665f0e9aefa9bd