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Structural basis for the association of PLEKHA7 with membrane-embedded phosphatidylinositol lipids
- Source :
- Structure
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- SummaryPLEKHA7 (pleckstrin homology domain containing family A member 7) plays key roles in intracellular signaling, cytoskeletal organization and cell adhesion, and is associated with multiple human cancers. The interactions of its pleckstrin homology (PH) domain with membrane phosphatidyl-inositol-phosphate (PIP) lipids, are critical for proper cellular localization and function, and their inhibition is an attractive target for anti-cancer therapy. While structural data can provide insights in this area, little is known about the way in which PLEKHA7 and other PH domains interact with membrane-embedded PIPs. Here we report atomic-resolution structures of the PLEHA7 PH domain and describe the molecular mechanism for its recognition of membrane-bound PIPs. Using X-ray crystallography, nuclear magnetic resonance (NMR), molecular dynamics (MD) simulations, and isothermal titration calorimetry (ITC), we show – in atomic-level detail – that the interaction of PLEKHA7 with PIPs is multivalent and induces PIP clustering. The PIP binding mechanism is distinct from a discrete one-to-one interaction. Our findings reveal a central role of the membrane assembly in mediating protein-PIP association and provide a roadmap for the design of PLEKHA7-PIP inhibitors.
- Subjects :
- Lipid Bilayers
Phosphatidylinositols
Article
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
PLEKHA7
Humans
Phosphatidylinositol
Cell adhesion
Molecular Biology
Nanodisc
Cellular localization
030304 developmental biology
0303 health sciences
Binding Sites
030302 biochemistry & molecular biology
Cell Membrane
Isothermal titration calorimetry
Pleckstrin homology domain
chemistry
Biophysics
lipids (amino acids, peptides, and proteins)
Carrier Proteins
Function (biology)
Intracellular
Protein Binding
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Structure
- Accession number :
- edsair.doi.dedup.....daea24b4164e0387a064f625df568f36
- Full Text :
- https://doi.org/10.1101/2020.11.25.387084