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A new insight into subinteractomes of functional antagonists: Thromboxane (CYP5A1) and prostacyclin (CYP8A1) synthases
- Source :
- Cell Biology International.
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The current article aims to summarize all possible spectrum of protein-protein interactions for thromboxane A synthase (CYP5A1) and prostacyclin synthase (CYP8A1). These enzymes metabolize the same substrate (prostaglandin H2 ) and can participate in cardiovascular, inflammatory, immune processes, and apoptosis modulation, as well as significantly influence the risk of cancers. Binary protein-protein and multiprotein complexes are of great importance in enzyme-regulating and signal-transduction pathways. However, protein partners of CYP5A1 and CYP8A1 are not yet fully identified, although both synthases are considered as prospective drug targets. At least 36 novel protein partners of CYP5A1 and CYP8A1 were revealed from different tissue types using an approach based on affinity isolation and mass spectrometry. Enrichment analysis showed that these proteins have different molecular functions: folding (refolding), unfolded protein and chaperon binding, protein transport (export/import), posttranslational modification, protein domain-specific binding, antioxidant activity, and glutathione homeostasis. A significant part of them, belonging to molecular chaperones, were common partners for CYP5A1 and CYP8A1, while other proteins were unique with the tissue-dependent distribution. New aspects of CYP5A1 and CYP8A1 interactomics and hetero-complex formation with different protein partners, including cytochrome P450s are discussed.
- Subjects :
- 0301 basic medicine
Cytochrome
Thromboxane
Prostacyclin
Ligands
Prostacyclin synthase
03 medical and health sciences
0302 clinical medicine
Cytochrome P-450 Enzyme System
medicine
Humans
chemistry.chemical_classification
biology
Cytochrome P450
Cell Biology
General Medicine
Transport protein
030104 developmental biology
Enzyme
chemistry
Biochemistry
Multiprotein Complexes
030220 oncology & carcinogenesis
biology.protein
Thromboxane-A Synthase
Thromboxane-A synthase
Protein Binding
medicine.drug
Subjects
Details
- ISSN :
- 10958355 and 10656995
- Database :
- OpenAIRE
- Journal :
- Cell Biology International
- Accession number :
- edsair.doi.dedup.....dad4b162958b8110b6973f5cacf1107d
- Full Text :
- https://doi.org/10.1002/cbin.11564